重组H5N1 HA蛋白

H5N1

人胚胎肾细胞（HEK293）

A DNA sequence encoding the extracellular domain of Influenza A virus (A/Turkey/1/2005 (H5N1)) (ABD73284.1) hemagglutinin (Met 1-Gln 531) with cleavage site mutated (GERRRKKR ? TETR, HA1+HA2, uncleaved) was expressed with a C-terminal polyhistidine tag.

> 95 % as determined by SDS-PAGE

The secreted recombinant Influenza A virus (A//Turkey/1/2005 (H5N1) HA (mutated) comprises 522 amino acids with the predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 65-70 kDa band in SDS-PAGE under reducing conditions.

SPNA-1; C-BAS/HAS; H-RASIDX; HAMSV; SPNA1; C-HA-RAS1; C-H-RAS; SPH; CTLO; HA; HRAS1; P21RAS; AI451697; IHJ; NMF4; AF093576; RASH1

Hemagglutinin

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.