重组人CA10蛋白

产品详情

  • 产品名称
    重组人CA10蛋白
  • 蛋白种属
    人
  • 表达宿主
    人胚胎肾细胞（HEK293）
  • Unitprot ID
    Q9NS85
  • 克隆构建
    A DNA sequence encoding the human CARPX (NP_001076002.1) (Met 1-Asn 300) was expressed with a C-terminal polyhistidine tag.
  • 蛋白纯度
    > 97 % as determined by SDS-PAGE
  • 蛋白活性
    Measured by its esterase activity. The specific activity is >10 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
  • 分子量
    The recombinant human CARPX consists of 291 amino acids and has a predicted molecular mass of 33 kDa. rhCARPX migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
  • 蛋白别名
    CA10; CARBONIC ANHYDRASE X; HUCEP-15; CAR10; BB085816; CARBONIC ANHYDRASE 10; 2700029L05RIK; CA-RPX; RP23-448I9.1; CARPX
  • 蛋白全名
    Carbonic anhydrase-related protein 10
  • 蛋白描述
    Carbonic anhydrase X, also called carbonic anhydrase - related protein X (CARPX) and CA10, belongs to the CA family of zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide in various biological processes such as respiration, renal tubular acidification and bone resorption. The secreted protein CARPX without CA activity (hydration of CO2) is identified as an acatalytic member of the alpha-carbonic anhydrase subgroup. CARP X expression is detected in the adult total brain and almost all parts of the central nervous system, but not in the fetal brain. Accordingly, CARP X is suggested to play a role in the development of central nervous system, especially the brain. The same CARP X protein are encoded by multiple transcript variants of this gene.
  • 发货形式
    Lyophilized from sterile PBS, pH 7.4
    1. 5 % trehalose and mannitol are added as protectants before lyophilization.
    2. Please contact us for any concerns or special requirements.
  • 内毒素
    < 1.0 EU per μg of the protein as determined by the LAL method
  • 保存条件
    Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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