重组大鼠ICAM1蛋白

产品详情

  • 产品名称
    重组大鼠ICAM1蛋白
  • 蛋白种属
    大鼠
  • 表达宿主
    人胚胎肾细胞（HEK293）
  • Unitprot ID
    Q00238
  • 蛋白序列
    Met 1 - Thr 493
  • 克隆构建
    A DNA sequence encoding the rat ICAM1 (Q00238-1) extracellular domain (Met 1-Thr 493) was expressed, fused with a polyhistidine tag at the C-terminus.
  • 蛋白纯度
    > 97 % as determined by SDS-PAGE
  • 蛋白活性
    Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.
    When cells are added to rat ICAM1 coated plates (12.5 µg/mL, 100 µL/well), approximately > 30% cells will adhere specifically.
  • 分子量
    The recombinant rat ICAM1 comprises 477 amino acids and predicts a molecular mass of 52.7 kDa. The apparent molecular mass of the rat ICAM1 is approximately 70-75 kDa in SDS-PAGE under reducing conditions due to glycosylation.
  • 蛋白别名
    MYD10; MALA-2; P3.58; CD54; LY-47; ICAM1; MGC6195; BB2; ICAM-1
  • 蛋白全名
    Intercellular adhesion molecule 1
  • 蛋白描述
    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
  • 发货形式
    Lyophilized from sterile PBS, pH 7.4
    1. 5 % trehalose and mannitol are added as protectants before lyophilization.
    2. Please contact us for any concerns or special requirements.
  • 内毒素
    < 1.0 EU per μg of the protein as determined by the LAL method
  • 保存条件
    Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
声明：本公司出售产品只能用于科研目的，不得用于诊断或者治疗！