• KLPGWSG polypeptide is a novel bioactive peptide with significant potential in neural tissue engineering. It was identified through Phage Display technique on murine neural stem cells (NSC) and was found to be present in proteins involved in stem cell maintenance and differentiation.
• This peptide binds to molecules on the cell surface of NSC and has been shown to enhance neuronal differentiation of NSC when added to the differentiation medium or linked to a self-assembling peptide (SAP) scaffold. When conjugated to a FITC fluorochrome, it can potentially be used as an NSC marker. The peptide does not substitute for or increase the proliferative effect of epidermal growth factor (EGF) on NSC and does not prevent cell death in the absence of EGF and basic fibroblast growth factor (bFGF). However, it positively modulates the neuronal differentiation of NSC, making it a promising candidate for biomimetic studies and stem cell therapies aimed at nervous system regeneration.
• The KLPGWSG-functionalized SAP scaffolds, such as Ac-KLP, with appropriate stiffness, support cell attachment and survival, and show enhanced neuronal differentiation compared to non-functionalized scaffolds, suggesting a synergistic effect of the peptide's presence and scaffold properties.

• Caprini A, Silva D, Zanoni I, Cunha C, Volontè C, Vescovi A, Gelain F. A novel bioactive peptide: assessing its activity over murine neural stem cells and its potential for neural tissue engineering. N Biotechnol. 2013 Jun 25;30(5):552-62. doi: 10.1016/j.nbt.2013.03.005. Epub 2013 Mar 26. PMID: 23541699.

三氟乙酸（TFA）是一种强酸，常用于从固相树脂中裂解合成的多肽，也可用于提高多肽纯化步骤中的HPLC性能。默认情况下，定制的多肽是以冻干的TFA盐的形式交付的，其中TFA的含量可能高达10-45%。

定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如，nM浓度的TFA已被证明会干扰细胞实验，在某些情况下会抑制细胞增殖，而在其他情况下会增加细胞活力。

TFA去除服务推荐用于:

• 将用于细胞试验的多肽
• 将被用作原料药或制成品的多肽
• 含有大量碱性残基的亲水多肽

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。