产品详情
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产品名称
Neurite Outgrowth-promoting Site 多肽
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序列
H-VFDNFVLK-OH
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三字母序列
H-Val-Phe-Asp-Asn-Phe-Val-Leu-Lys-OH
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长度 (aa)
8
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多肽纯度 (HPLC, %)
95.5
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分子式
C48H72N10O12
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分子量(Da)
981.14
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来源
固相合成
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描述
FPGERGVEGPGP polypeptide is an eight-amino acid sequence within the alternatively spliced FN - III repeat D of human tenascin - C. It has been identified as crucial for neurite outgrowth promotion. This peptide is active by itself and also functions in the context of the large tenascin - C splice variant. The biological activity of FPGERGVEGPGP depends on specific amino acids within the sequence.
It can overcome the inhibitory effect of chondroitin sulfate proteoglycans (CSPGs) on neurite outgrowth in vitro, which is significant as CSPGs are upregulated in glial scars and impede axonal regrowth. In neural tissue engineering applications, it has the potential to enhance neuronal regeneration and differentiation. For example, in studies with PC12 cells, related peptide nanofibers have been shown to increase the length and number of neurites in 2D and 3D cultures, and promote the expression of neural markers such as β - III tubulin and synaptophysin, suggesting its possible role in the development of therapies for neurological conditions. -
储存指南
一般情况下， Neurite Outgrowth-promoting Site 多肽 粉末应该保存在-20甚至-70摄氏度. Neurite Outgrowth-promoting Site 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南
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参考文献
- Sever M , Gunay G , Guler MO , Tekinay AB . Tenascin-C derived signaling induces neuronal differentiation in a three-dimensional peptide nanofiber gel. Biomater Sci. 2018 Jun 25;6(7):1859-1868. doi: 10.1039/c7bm00850c. PMID: 29799029.
- Mercado ML, Nur-e-Kamal A, Liu HY, Gross SR, Movahed R, Meiners S. Neurite outgrowth by the alternatively spliced region of human tenascin-C is mediated by neuronal alpha7beta1 integrin. J Neurosci. 2004 Jan 7;24(1):238-47. doi: 10.1523/JNEUROSCI.4519-03.2004. PMID: 14715956; PMCID: PMC6729556.
- Meiners S, Nur-e-Kamal MS, Mercado ML. Identification of a neurite outgrowth-promoting motif within the alternatively spliced region of human tenascin-C. J Neurosci. 2001 Sep 15;21(18):7215-25. doi: 10.1523/JNEUROSCI.21-18-07215.2001. PMID: 11549732; PMCID: PMC6762977.
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关于三氟乙酸盐
三氟乙酸（TFA）是一种强酸，常用于从固相树脂中裂解合成的多肽，也可用于提高多肽纯化步骤中的HPLC性能。默认情况下，定制的多肽是以冻干的TFA盐的形式交付的，其中TFA的含量可能高达10-45%。
定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如，nM浓度的TFA已被证明会干扰细胞实验，在某些情况下会抑制细胞增殖，而在其他情况下会增加细胞活力。
TFA去除服务推荐用于:
- 将用于细胞试验的多肽
- 将被用作原料药或制成品的多肽
- 含有大量碱性残基的亲水多肽
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关于多肽净含量
干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。
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摩尔浓度计算器
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稀释计算器
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百分比浓度计算器
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
相关服务
多肽合成服务: 纽普生物（NovoPro）提供常规多肽合成、糖修饰多肽合成、稳定同位素标记的多肽合成、磷酸化多肽合成、荧光标记多肽、多肽的BSA/KLH/OVA等载体蛋白的偶联等服务。