我们所展示的质粒图谱主要是从文献和开放数据库中收集而来,主要是为了方便研究工作,其中一小部分质粒进行了质量控制,可供科学家使用。
所有的产品都严格仅供科学研究使用,不能应用于临床试验,包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确,但是我们并不能保证实验结果。页面展示的图谱序列为理论序列,可能与测序结果不一致,请自行比对后确定是否满足要求。(如果测过序,本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%,则将其视为正确。
由于科学研究是在探索未知,具有很大的不确定性,在任何情况下,我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pRS305
- 载体抗性:
- Ampicillin
- 载体长度:
- 5504 bp
- 载体类型:
- Yeast Plasmids
- 复制子:
- ori
- 宿主:
- Yeast
- 载体来源:
- Sikorski RS, Hieter P.
- 拷贝数:
- High copy number
- 启动子:
- LEU2
- 5'引物:
- M13 fwd
- 3'引物:
- M13 rev
pRS305 载体载体图谱
pRS305 载体载体序列
LOCUS 40924_37793 5504 bp DNA circular SYN 01-JAN-1980 DEFINITION Yeast integrative vector with a LEU2 marker and an MCS derived from pBLUESCRIPT. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5504) AUTHORS Sikorski RS, Hieter P. TITLE A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. JOURNAL Genetics 1989;122:19-27. PUBMED 2659436 REFERENCE 2 (bases 1 to 5504) TITLE Direct Submission REFERENCE 3 (bases 1 to 5504) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; date: "1989"; volume: "122"; pages: "19-27" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..5504 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(666..1757) /codon_start=1 /label=LEU2 /note="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" promoter complement(1770..2174) /label=LEU2 promoter rep_origin complement(2474..2929) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 3074..3090 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 3097..3115 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 3124..3231 /label=MCS /note="pBluescript multiple cloning site" promoter complement(3244..3262) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(3283..3299) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3307..3323) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3331..3361) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3376..3397) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3685..4273) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4447..5304) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5305..5409) /label=AmpR promoter
我们不提供该载体,载体信息仅供查询和参考。