- 载体名称:
- pET-27b(+)
- 载体抗性:
- Kanamycin
- 载体长度:
- 5414 bp
- 载体类型:
- pET & Duet Vectors (Novagen)
- 复制子:
- ori
- 载体来源:
- Novagen (EMD Millipore)
- 拷贝数:
- High copy number
pET-27b(+) 载体载体图谱
pET-27b(+) 载体载体序列
LOCUS pET-27b(+). 5414 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector with a signal sequence and a kanamycin resistance marker for expression of C-terminally HSV-tagged proteins in the periplasm. ACCESSION . VERSION . KEYWORDS pET-27b(+). SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5414) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 5414) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5414 /mol_type="other DNA" /organism="synthetic DNA construct" terminator complement(26..73) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(140..157) /label=6xHis /note="6xHis affinity tag" CDS complement(164..196) /label=HSV tag /note="HSV (herpes simplex virus) epitope tag" misc_feature 212..279 /label=MCS /note="MCS" /note="multiple cloning site" sig_peptide complement(278..343) /label=pelB signal sequence /note="leader peptide for secretion" RBS complement(351..373) /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(388..412) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(413..431) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 740..817 /label=lacI promoter CDS 818..1897 /label=lacI /note="lac repressor" protein_bind 1913..1934 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS 2709..2897 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 3002..3144 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(3330..3918) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS 4040..4852 /label=KanR /note="aminoglycoside phosphotransferase" rep_origin complement(4948..5403) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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