我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。

所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。

确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)

开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。

由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。

下载GeneBank文件(.gb)

LOCUS       pBACgus4x-1.        8066 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer plasmid encoding beta-glucuronidase, with two 
            polyhedrin and two p10 promoters, for the simultaneous expression of
            up to four proteins.
ACCESSION   .
VERSION     .
KEYWORDS    pBACgus4x-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     329..1156
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             complement(1170..2978)
                     /label=GUS
                     /note="beta-glucuronidase"
     promoter        complement(2987..3302)
                     /label=p6.9 promoter
                     /note="baculovirus late basic protein promoter"
     polyA_signal    complement(3320..3368)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3371..3397
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(3400..3509)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    3642..3652
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(3660..3751)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        3776..3885
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    3893..3904
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    3905..3953
                     /label=poly(A) signal
                     /note="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     promoter        3991..4082
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     misc_feature    4086..4115
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             4116..4133
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_recomb     4170..5527
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(5790..6378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6552..7409)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7410..7514)
                     /label=AmpR promoter
     rep_origin      7541..7996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"