pAcGP67-B 载体 (V011683#)

我们所展示的质粒图谱主要是从文献和开放数据库中收集而来,主要是为了方便研究工作,其中一小部分质粒进行了质量控制,可供科学家使用。

所有的产品都严格仅供科学研究使用,不能应用于临床试验,包括人体摄入、注射或外用的药理学用途。

确保质粒的关键元件正确,但是我们并不能保证实验结果。页面展示的图谱序列为理论序列,可能与测序结果不一致,请自行比对后确定是否满足要求。(如果测过序,本页面一般会提供下载)

开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%,则将其视为正确。

由于科学研究是在探索未知,具有很大的不确定性,在任何情况下,我们都不承担超出质粒本身的额外经济损失或责任。

载体名称:
pAcGP67-B
载体抗性:
Ampicillin
载体长度:
9765 bp
载体类型:
Insect Cell Vectors
复制子:
ori
载体来源:
BD Biosciences
拷贝数:
High copy number
启动子:
polyhedrin

pAcGP67-B 载体载体图谱

pAcGP67-B9765 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600Tyrosine-protein phosphatasePrimase-associated factor LEF-2polyhedrin promoterGP64 signal sequenceMCScontains part of ORF1629M13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterM13 fwd

pAcGP67-B 载体载体序列

LOCUS       V011683                 9765 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011683
VERSION     V011683
KEYWORDS    pAcGP67-B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label="Tyrosine-protein phosphatase"
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     4189..4248
                     /label="GP64 signal sequence"
                     /note="signal sequence from the baculovirus envelope
                     glycoprotein GP64"
     misc_feature    4258..4318
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     4780..6235
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(7146..7162)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7170..7186)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7194..7224)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7239..7260)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7548..8136)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8310..9167)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9168..9272)
                     /label="AmpR promoter"
     primer_bind     9746..9762
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"

我们不提供该载体,载体信息仅供查询和参考。