- 载体名称:
- pAcGHLT B
- 载体抗性:
- Ampicillin
- 载体长度:
- 8755 bp
- 载体类型:
- Insect Cell Vectors
- 复制子:
- ori
- 载体来源:
- BD Biosciences
- 拷贝数:
- High copy number
- 启动子:
- polyhedrin
pAcGHLT B 载体载体图谱
pAcGHLT B 载体载体序列
LOCUS pAcGHLT_B. 8755 bp DNA circular SYN 01-JAN-1980 DEFINITION Baculovirus transfer vector for expressing proteins with an N-terminal GST-6xHis-PKA-thrombin cassette. For other reading frames, use pAcGHLT A or pAcGHLT C. ACCESSION . VERSION . KEYWORDS pAcGHLT B. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8755) AUTHORS BD Biosciences TITLE Direct Submission REFERENCE 2 (bases 1 to 8755) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..8755 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 761..1390 /gene="LEF-2" /label=LEF-2 /note="Primase-associated factor LEF-2 from Autographa californica nuclear polyhedrosis virus. Accession#: P41418" misc_recomb 1269..2096 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" promoter 2100..2191 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" CDS 2206..2859 /label=GST /note="glutathione S-transferase from Schistosoma japonicum" CDS 2875..2892 /label=6xHis /note="6xHis affinity tag" CDS 2899..2916 /codon_start=1 /product="recognition site for phosphorylation by protein kinase A" /label=recognition site for phosphorylation by protein /note="PKA site" /translation="RRASVA" CDS 2926..2943 /label=thrombin site /note="thrombin recognition and cleavage site" misc_feature 2966..3027 /label=MCS /note="MCS" /note="multiple cloning site" misc_recomb 3047..4505 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" primer_bind complement(5649..5665) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 5878..6333 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6615..6719 /label=AmpR promoter CDS 6720..7577 /label=AmpR /note="beta-lactamase" rep_origin 7751..8339 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 8627..8648 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 8663..8693 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8701..8717 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8725..8741 /label=M13 rev /note="common sequencing primer, one of multiple similar variants"
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