- 载体名称:
- pEGFP-C1
- 载体抗性:
- Kanamycin
- 载体长度:
- 4731 bp
- 载体类型:
- Fluorescent Protein Genes & Plasmids
- 复制子:
- ori
- 载体来源:
- Clontech
- 筛选标记:
- Neomycin/G418(Geneticin)
- 拷贝数:
- High copy number
- 启动子:
- CMV
- 克隆方法:
- Enzyme Cut
- 表达方法:
- Transient
pEGFP-C1 载体载体图谱
pEGFP-C1 载体载体序列
LOCUS pEGFP-C1. 4731 bp DNA circular SYN 01-JAN-1980
DEFINITION Vector for fusing EGFP to the N-terminus of a partner protein. For
other reading frames, use pEGFP-C2 or pEGFP-C3.
ACCESSION .
VERSION .
KEYWORDS pEGFP-C1.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4731)
AUTHORS Clontech
TITLE Direct Submission
REFERENCE 2 (bases 1 to 4731)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4731
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 613..1329
/label=EGFP
/note="enhanced GFP"
misc_feature 1330..1395
/label=MCS
/note="multiple cloning site"
misc_feature 1404..1414
/label=stop codons
/note="stop codons"
/note="stop codons in all three reading frames"
polyA_signal 1519..1640
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1647..2102)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2129..2233
/label=AmpR promoter
promoter 2235..2592
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 2627..3418
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 3653..3700
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4029..4617
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
我们不提供该载体，载体信息仅供查询和参考。