- 载体名称:
- pcDNA3.1(+)
- 载体抗性:
- Ampicillin
- 载体长度:
- 5428 bp
- 载体类型:
- Mammalian Expression Vectors
- 复制子:
- ori
- 载体来源:
- Invitrogen (Life Technologies)
- 筛选标记:
- Neomycin/G418(Geneticin)
- 拷贝数:
- High copy number
- 启动子:
- SV40
- 3'引物:
- M13 rev
pcDNA3.1(+) 载体载体图谱
pcDNA3.1(+) 载体载体序列
LOCUS pcDNA3.1(+). 5428 bp DNA circular SYN 01-JAN-1980 DEFINITION Mammalian expression vector with the CMV promoter. The MCS is in the forward (+) orientation. ACCESSION . VERSION . KEYWORDS pcDNA3.1(+). SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5428) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission REFERENCE 2 (bases 1 to 5428) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT The cloned gene must include a Kozak sequence and start codon. FEATURES Location/Qualifiers source 1..5428 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 895..1002 /label=MCS /note="MCS" /note="multiple cloning site" polyA_signal 1028..1252 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1298..1726 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 1740..2069 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 2136..2927 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" polyA_signal 3104..3237 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3274..3290) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(3298..3314) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3322..3352) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(3367..3388) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(3676..4261) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4435..5292) /label=AmpR /note="beta-lactamase" promoter complement(5293..5397) /label=AmpR promoter
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