我们所展示的质粒图谱主要是从文献和开放数据库中收集而来,主要是为了方便研究工作,其中一小部分质粒进行了质量控制,可供科学家使用。
所有的产品都严格仅供科学研究使用,不能应用于临床试验,包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确,但是我们并不能保证实验结果。页面展示的图谱序列为理论序列,可能与测序结果不一致,请自行比对后确定是否满足要求。(如果测过序,本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%,则将其视为正确。
由于科学研究是在探索未知,具有很大的不确定性,在任何情况下,我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pMpGWB136
- 载体抗性:
- Streptomycin
- 载体长度:
- 13637 bp
- 载体类型:
- Binary vector
- 复制子:
- ori
- 载体来源:
- Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, Shikanai T, Kohchi T.
- 启动子:
- CaMV35S(enhanced)
pMpGWB136 载体载体图谱
pMpGWB136 载体载体序列
LOCUS 40924_31455 13637 bp DNA circular SYN 18-DEC-2018 DEFINITION Binary vector pMpGWB136 DNA, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13637) AUTHORS Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, Shikanai T, Kohchi T. TITLE Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha JOURNAL PLoS ONE 10 (9), E0138876 (2015) PUBMED 26406247 REFERENCE 2 (bases 1 to 13637) AUTHORS Nishihama R, Ishizaki K, Kohchi T. TITLE Direct Submission JOURNAL Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/ REFERENCE 3 (bases 1 to 13637) TITLE Direct Submission REFERENCE 4 (bases 1 to 13637) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; date: "2015"; volume: "10"; issue: "9"; pages: "E0138876" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku, Kyoto, Kyoto 606-8502, Japan URL :http"; pages: "//www.lif.kyoto-u.ac.jp/e" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..13637 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature complement(54..78) /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" primer_bind 281..297 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" regulatory 307..2035 /label=MpEF1alpha promoter and 5'UTR /note="MpEF1alpha promoter and 5'UTR" /regulatory_class="promoter" CDS 2050..2763 /label=TagRFP-T /note="photostable variant of TagRFP (Shaner et al., 2008)" protein_bind 2749..2873 /label=attR1 /note="recombination site for the Gateway(R) LR reaction" promoter 2910..2940 /label=lac UV5 promoter /note="E. coli lac promoter with an 'up' mutation" CDS 2994..3650 /label=CmR /note="chloramphenicol acetyltransferase" CDS 3995..4297 /label=ccdB /note="CcdB, a bacterial toxin that poisons DNA gyrase" protein_bind complement(4341..4465) /label=attR2 /note="recombination site for the Gateway(R) LR reaction" terminator 4497..4749 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(4783..4799) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 4807..4823 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4831..4861) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4876..4897) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." polyA_signal complement(4982..5156) /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(5200..6222) /label=HygR /note="aminoglycoside phosphotransferase from E. coli" promoter complement(6289..6964) /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" misc_feature complement(7159..7183) /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 7704..8492 /label=SmR /note="aminoglycoside adenylyltransferase (Murphy, 1985)" rep_origin 8741..9329 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(9515..9655) /label=bom /note="basis of mobility region from pBR322" rep_origin complement(9999..10193) /direction=LEFT /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(10262..11332) /label=pVS1 RepA /note="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(11764..12390) /label=pVS1 StaA /note="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)"
我们不提供该载体,载体信息仅供查询和参考。