下载GeneBank文件(.gb)

LOCUS       62056_7965       16805 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16805)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..16805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(63..96)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     primer_bind     complement(566..582)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(852..1440)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1565..2422)
                     /label=AmpR
                     /note="beta-lactamase"
     protein_bind    2807..2840
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     rep_origin      3168..8388
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans
                     (Aleksenko and Clutterbuck, 1996)"
     promoter        complement(8695..8713)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(8946..9752)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             10899..14798
                     /gene="cas12a"
                     /label=cas12a
                     /note="CRISPR-associated endonuclease Cas12a from
                     Francisella tularensis subsp. novicida (strain U112). 
                     Accession#: A0Q7Q2"
     CDS             14811..14831
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             15665..16036
                     /label=BleoR
                     /note="antibiotic-binding protein"