pEBTet_NAD-Snifit-nucl 载体 (V017001#)

我们所展示的质粒图谱主要是从文献和开放数据库中收集而来,主要是为了方便研究工作,其中一小部分质粒进行了质量控制,可供科学家使用。

所有的产品都严格仅供科学研究使用,不能应用于临床试验,包括人体摄入、注射或外用的药理学用途。

确保质粒的关键元件正确,但是我们并不能保证实验结果。页面展示的图谱序列为理论序列,可能与测序结果不一致,请自行比对后确定是否满足要求。(如果测过序,本页面一般会提供下载)

开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%,则将其视为正确。

由于科学研究是在探索未知,具有很大的不确定性,在任何情况下,我们都不承担超出质粒本身的额外经济损失或责任。

载体名称:
pEBTet_NAD-Snifit-nucl
载体抗性:
Ampicillin
载体长度:
13899 bp
载体类型:
Protein expression
复制子:
ori
宿主:
Mammalian cells
筛选标记:
Puro
启动子:
RSV

pEBTet_NAD-Snifit-nucl 载体载体图谱

pEBTet_NAD-Snifit-nucl13899 bp60012001800240030003600420048005400600066007200780084009000960010200108001140012000126001320013800Sepiapterin reductaseHaloTag(R)SNAP-tag(R)SV40 NLSSV40 NLSbGH poly(A) signalAmpR promoterAmpRoriSV40 promoterPuroRSV40 poly(A) signalRSV promoterbeta-globin intronT7 promoterTetRCMV enhancerCMV promotertet operator

pEBTet_NAD-Snifit-nucl 载体载体序列

LOCUS       V017001                13899 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017001
VERSION     V017001
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             12..794
                     /gene="SPR"
                     /label="Sepiapterin reductase"
                     /note="Sepiapterin reductase from Homo sapiens. Accession#:
                     P35270"
     CDS             804..1691
                     /label="HaloTag(R)"
                     /note="modified bacterial dehalogenase that forms covalent
                     bonds with chloroalkane derivatives"
     CDS             1815..2360
                     /label="SNAP-tag(R)"
                     /note="human O6-alkylguanine-DNA-alkyltransferase variant
                     that forms covalent bonds with benzylguanine derivatives"
     CDS             2373..2393
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2397..2417
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2530..2641
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        7721..7825
                     /label="AmpR promoter"
     CDS             7826..8683
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8857..9445
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        9652..9949
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             10026..10622
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     polyA_signal    10716..10850
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        11284..11545
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          11662..12234
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     promoter        12289..12307
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             12318..12938
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     enhancer        13156..13535
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13536..13739
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    13741..13759
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"

我们不提供该载体,载体信息仅供查询和参考。