下载GeneBank文件(.gb)

LOCUS       40924_46343        8783 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pWCD2139, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE.
  TITLE     An enzyme-coupled biosensor enables (S)-reticuline production in 
            yeast from glucose
  JOURNAL   Nat. Chem. Biol. 11 (7), 465-471 (2015)
  PUBMED    25984720
REFERENCE   2  (bases 1 to 8783)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJJ., Dueber 
            JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2015) Department of Bioengineering, University of 
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA 
            94704, USA
REFERENCE   3  (bases 1 to 8783)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8783)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem. 
            Biol."; date: "2015"; volume: "11"; issue: "7"; pages: "465-471"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2015) Department of Bioengineering, University of 
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA 
            94704, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..524
                     /label=similar to URA3 5'
                     /note="similar to URA3 5'"
     promoter        586..1252
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     CDS             1259..2749
                     /gene="CYP76AD1"
                     /label=CYP76AD1
                     /note="Cytochrome P450 76AD1 from Beta vulgaris.
                     Accession#: I3PFJ5"
     regulatory      2765..2988
                     /regulatory_class="terminator"
     regulatory      3022..3721
                     /regulatory_class="promoter"
     CDS             3728..5146
                     /codon_start=1
                     /product="DOPA decarboxylase"
                     /label=DOPA decarboxylase
                     /note="PpDODC (DOPA decarboxylase) derived from Pseudomonas
                     putida"
                     /protein_id="AKH61544.1"
                     /translation="MTPEQFRQYGHQLIDLIADYRQTVGERPVMAQVEPGYLKAALPAT
                     APQQGEPFAAILDDVNNLVMPGLSHWQHPDFYGYFPSNGTLSSVLGDFLSTGLGVLGLS
                     WQSSPALSELEETTLDWLRQLLGLSGQWSGVIQDTASTSTLVALISARERATDYALVRG
                     GLQAEPKPLIVYVSAHAHSSVDKAALLAGFGRDNIRLIPTDERYALRPEALQAAIEQDL
                     AAGNQPCAVVATTGTTTTTALDPLRPVGEIAQANGLWLHVDSAMAGSAMILPECRWMWD
                     GIELADSVVVNAHKWLGVAFDCSIYYVRDPQHLIRVMSTNPSYLQSAVDGEVKNLRDWG
                     IPLGRRFRALKLWFMLRSEGVDALQARLRRDLDNAQWLAGQVEAAAEWEVLAPVQLQTL
                     CIRHRPAGLEGEALDAHTKGWAERLNASGAAYVTPATLDGRWMVRVSIGALPTERGDVQ
                     RLWARLQDVIKGGS"
     terminator      5153..5340
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     regulatory      5426..5602
                     /regulatory_class="promoter"
     CDS             5603..6403
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      6407..6484
                     /regulatory_class="terminator"
     CDS             6674..6934
                     /gene="TIM9"
                     /label=TIM9
                     /note="Mitochondrial import inner membrane translocase
                     subunit TIM9 from Saccharomyces cerevisiae (strain ATCC 
                     204508 / S288c). Accession#: O74700"
     regulatory      complement(7021..7047)
                     /regulatory_class="terminator"
     CDS             complement(7051..7863)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(7864..8011)
                     /regulatory_class="promoter"
     rep_origin      8088..8676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"