我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。

所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。

确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)

开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。

由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。

下载GeneBank文件(.gb)

LOCUS       40924_5064        6572 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pAttR-YFP-2xHA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Conn SJ.
  TITLE     Magnesium transporters in Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Conn SJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 6572)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6572)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-JUL-2010) Agriculture, Food "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..756
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     regulatory      756..898
                     /note="translational enhancer; 5' UTR from tobacco etch
                     virus"
                     /regulatory_class="enhancer"
     protein_bind    904..1028
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1053..1083
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1137..1793
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2138..2440
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2484..2608)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2610..3326
                     /label=EYFP
                     /note="enhanced YFP"
     CDS             3336..3362
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             3372..3398
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     promoter        complement(3683..3701)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3722..3738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3746..3762)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3770..3800)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3815..3836)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4124..4712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4886..5743)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5744..5848)
                     /label=AmpR promoter
     rep_origin      complement(5874..6329)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6471..6487
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6494..6512
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6537..6553
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"