pMXs-IRES-BFP 载体图谱和序列

载体名称:: pMXs-IRES-BFP

载体抗性:: Ampicillin

载体长度:: 5942 bp

载体类型:: Retroviral vectors

复制子:: ori

筛选标记:: TagBFP

5'引物:: pBABE 5': CTTTATCCAGCCCTCAC

3'引物:: pBABE 3': ACCCTAACTGACACACATTCC

pMXs-IRES-BFP 载体载体图谱

pMXs-IRES-BFP 载体载体序列

Copy Sequence

下载GeneBank文件(.gb)

LOCUS       40924_32888        5942 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5942)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     misc_feature    1992..2577
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             2578..3276
                     /label=TagBFP
                     /note="monomeric blue fluorescent protein"
     LTR             3325..3916
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(4217..4805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4972..5829)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5830..5934)
                     /label=AmpR promoter

我们不提供该载体，载体信息仅供查询和参考。