我们所展示的质粒图谱主要是从文献和开放数据库中收集而来,主要是为了方便研究工作,其中一小部分质粒进行了质量控制,可供科学家使用。
所有的产品都严格仅供科学研究使用,不能应用于临床试验,包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确,但是我们并不能保证实验结果。页面展示的图谱序列为理论序列,可能与测序结果不一致,请自行比对后确定是否满足要求。(如果测过序,本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%,则将其视为正确。
由于科学研究是在探索未知,具有很大的不确定性,在任何情况下,我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pBACgus4x-1
- 载体抗性:
- Ampicillin
- 载体长度:
- 8066 bp
- 载体类型:
- Insect Cell Vectors
- 复制子:
- ori
- 载体来源:
- Novagen (EMD Millipore)
- 拷贝数:
- High copy number
- 启动子:
- p6.9
pBACgus4x-1 载体载体图谱
pBACgus4x-1 载体载体序列
LOCUS pBACgus4x-1. 8066 bp DNA circular SYN 01-JAN-1980 DEFINITION Baculovirus transfer plasmid encoding beta-glucuronidase, with two polyhedrin and two p10 promoters, for the simultaneous expression of up to four proteins. ACCESSION . VERSION . KEYWORDS pBACgus4x-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8066) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 8066) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..8066 /mol_type="other DNA" /organism="synthetic DNA construct" misc_recomb 329..1156 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" CDS complement(1170..2978) /label=GUS /note="beta-glucuronidase" promoter complement(2987..3302) /label=p6.9 promoter /note="baculovirus late basic protein promoter" polyA_signal complement(3320..3368) /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 3371..3397 /label=MCS /note="MCS" /note="multiple cloning site" promoter complement(3400..3509) /label=p10 promoter /note="baculovirus promoter for expression in insect cells" misc_feature 3642..3652 /label=MCS /note="MCS" /note="multiple cloning site" promoter complement(3660..3751) /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" promoter 3776..3885 /label=p10 promoter /note="baculovirus promoter for expression in insect cells" misc_feature 3893..3904 /label=MCS /note="MCS" /note="multiple cloning site" misc_feature 3905..3953 /label=poly(A) signal /note="poly(A) signal" /note="synthetic polyadenylation signal" promoter 3991..4082 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" misc_feature 4086..4115 /label=MCS /note="MCS" /note="multiple cloning site" CDS 4116..4133 /label=6xHis /note="6xHis affinity tag" misc_recomb 4170..5527 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" rep_origin complement(5790..6378) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6552..7409) /label=AmpR /note="beta-lactamase" promoter complement(7410..7514) /label=AmpR promoter rep_origin 7541..7996 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
我们不提供该载体,载体信息仅供查询和参考。