我们所展示的质粒图谱主要是从文献和开放数据库中收集而来,主要是为了方便研究工作,其中一小部分质粒进行了质量控制,可供科学家使用。
所有的产品都严格仅供科学研究使用,不能应用于临床试验,包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确,但是我们并不能保证实验结果。页面展示的图谱序列为理论序列,可能与测序结果不一致,请自行比对后确定是否满足要求。(如果测过序,本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%,则将其视为正确。
由于科学研究是在探索未知,具有很大的不确定性,在任何情况下,我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pCLIP-Cas9-Nuclease-hCMV-Puro
- 载体抗性:
- Ampicillin
- 载体长度:
- 11059 bp
- 载体类型:
- CRISPR Plasmids
- 复制子:
- ori
- 载体来源:
- transOMIC
- 拷贝数:
- High copy number
- 启动子:
- CMV
pCLIP-Cas9-Nuclease-hCMV-Puro 载体载体图谱
pCLIP-Cas9-Nuclease-hCMV-Puro 载体载体序列
LOCUS V012233 11059 bp DNA circular SYN 01-JAN-1980 DEFINITION Exported. ACCESSION V012233 VERSION V012233 KEYWORDS pCLIP-Cas9-Nuclease-hCMV-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 11059) AUTHORS transOMIC TITLE Direct Submission REFERENCE 2 (bases 1 to 11059) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..11059 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 27..404 /label="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" LTR 411..668 /label="5' LTR (truncated)" /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 715..840 /label="HIV-1 Psi" /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1333..1566 /label="RRE" /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1751..1795 /label="gp41 peptide" /note="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" CDS 1944..1985 /note="Protein Tat from Human immunodeficiency virus type 1 group M subtype B (isolate WMJ22). Accession#: P12509" /label="Protein Tat" misc_feature 2057..2174 /label="cPPT/CTS" /note="central polypurine tract and central termination sequence of HIV-1" enhancer 2238..2541 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 2542..2745 /label="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 2905..7008 /label="Cas9" /note="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" CDS 7009..7056 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label="bipartite nuclear localization signal from nucl" /note="nucleoplasmin NLS" /translation="KRPAATKKAGQAKKKK" CDS 7057..7080 /label="FLAG" /note="FLAG(R) epitope tag, followed by an enterokinase cleavage site" CDS 7090..7146 /label="P2A" /note="2A peptide from porcine teschovirus-1 polyprotein" CDS 7147..7740 /label="PuroR" /note="puromycin N-acetyltransferase" misc_feature 7768..8356 /label="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" LTR 8561..8794 /label="3' LTR (Delta-U3)" /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" promoter 8890..9219 /label="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin complement(9308..9893) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(10067..10924) /label="AmpR" /note="beta-lactamase" promoter complement(10925..11029) /label="AmpR promoter"
我们不提供该载体,载体信息仅供查询和参考。