我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。
所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。
由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pJG030
- 载体抗性:
- Ampicillin
- 载体长度:
- 5607 bp
- 载体类型:
- Cloning vector
- 复制子:
- ori
- 载体来源:
- Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
pJG030 载体图谱
质粒操作方法
1. 发货形式：质粒干粉(常温运输，存于-20度，请务必先转化提质粒后使用)
2. 收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O溶解质粒;（质粒复测的浓度有时候与标称值差距较大，这可能是因为冻干质粒在管中的位置、复溶效率、测量偏差以及管壁的吸附导致，因此建议先转化提质粒后再使用）
3. 取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；
4. 加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min (30℃培养1-1.5小时);
5. 6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上;
6. 将平板倒置37℃培养14h，如果要求是30℃则培养20h; (菌落过多则将质粒稀释后再转化；没有菌落则加入10μl质粒转化；建议不要直接转表达感受态， 要先转克隆感受态，重提质粒后再导入表达感受态);
7. 挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
pJG030 载体序列
LOCUS V005343 5607 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V005343
VERSION V005343
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 5607)
AUTHORS Goodson J, Klupt S, Zhang C, Straight P, Winkler W.
TITLE A Broadly Conserved Antiterminator Protein Controls a Regulon of
Bacillus amyloliquefaciens Antibiotic Gene Clusters
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5607)
AUTHORS Goodson JR.
TITLE Direct Submission
JOURNAL Submitted (01-JUL-2016) Cell Biology and Molecular Genetics,
University of Maryland, 3112 Bioscience Research Building, College
Park, MD 20742, USA
REFERENCE 3 (bases 1 to 5607)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5607)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(01-JUL-2016) Cell Biology and Molecular Genetics, University of
Maryland, 3112 Bioscience Research Building, College Park, MD 20742,
USA"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5607
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 379..395
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 397..1294
/note="LoaP region homology; homology region upstream of
loaP containing dfnA leader"
gene complement(397..528)
/pseudo=""
/gene="dfnA"
/label="dfnA"
gene 1262..1294
/pseudo=""
/gene="loaP"
/label="loaP"
/note="5'"
CDS 1745..2476
/gene="ermC"
/label="rRNA adenine N-6-methyltransferase"
/note="rRNA adenine N-6-methyltransferase from
Staphylococcus aureus. Accession#: P02979"
misc_feature 2573..3342
/note="LoaP Region Homology; homology region downstream of
loaP"
gene 2573..2603
/pseudo=""
/gene="loaP"
/label="loaP"
/note="3'"
gene complement(2947..3342)
/pseudo=""
/locus_tag="RBA_022100"
/label="RBA_022100"
misc_feature 3343..3373
/note="MCS; pUC19 multiple cloning site"
primer_bind complement(3386..3402)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3410..3426)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3434..3464)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(3479..3500)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3788..4376)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4550..5407)
/label="AmpR"
/note="beta-lactamase"
promoter complement(5408..5512)
/label="AmpR promoter"