我们所展示的质粒图谱主要是从文献和开放数据库中收集而来,主要是为了方便研究工作,其中一小部分质粒进行了质量控制,可供科学家使用。
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开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%,则将其视为正确。
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- 载体名称:
- pCMV-Twinstrep-NK1R-mEGFP-FLAG-10×His-mCherry
- 载体抗性:
- Ampicillin
- 载体长度:
- 9536 bp
- 载体类型:
- Protein expression
- 复制子:
- ori
- 宿主:
- Mammalian cells
- 启动子:
- CMV
pCMV-Twinstrep-NK1R-mEGFP-FLAG-10×His-mCherry 载体载体图谱
pCMV-Twinstrep-NK1R-mEGFP-FLAG-10×His-mCherry 载体载体序列
LOCUS 62056_7625 9536 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9536) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..9536 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 102..556 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 582..686 /label=AmpR promoter CDS 687..1544 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1718..2306 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" mobile_element complement(2611..2835) /label=Tn7R /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(2905..3435) /codon_start=1 /label=GmR /note="gentamycin acetyltransferase" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3624..3652) /label=Pc promoter /note="class 1 integron promoter" polyA_signal complement(4169..4217) /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" CDS complement(4291..4998) /codon_start=1 /label=mCherry /note="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" promoter complement(5031..5140) /label=p10 promoter /note="baculovirus promoter for expression in insect cells" promoter 5185..5276 /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" enhancer 5457..5835 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 5836..6039 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 6175..6307 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" CDS 6419..6442 /codon_start=1 /label=Strep-Tag II /note="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /translation="WSHPQFEK" CDS 6479..6502 /codon_start=1 /label=Strep-Tag II /note="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /translation="WSHPQFEK" CDS 7565..7585 /codon_start=1 /label=TEV site /note="tobacco etch virus (TEV) protease recognition and cleavage site" /translation="ENLYFQG" CDS 7604..8320 /codon_start=1 /label=mEGFP /note="enhanced GFP with monomerizing A206K mutation (Zacharias et al., 2002)" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 8321..8344 /codon_start=1 /label=FLAG /note="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /translation="DYKDDDDK" CDS 8345..8371 /codon_start=1 /label=9xHis /note="9xHis affinity tag" /translation="HHHHHHHHH" misc_feature 8405..8993 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" polyA_signal 9126..9260 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" mobile_element complement(9289..9454) /label=Tn7L /note="mini-Tn7 element (left end of the Tn7 transposon)"
我们不提供该载体,载体信息仅供查询和参考。