我们所展示的质粒图谱主要是从文献和开放数据库中收集而来,主要是为了方便研究工作,其中一小部分质粒进行了质量控制,可供科学家使用。
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开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%,则将其视为正确。
由于科学研究是在探索未知,具有很大的不确定性,在任何情况下,我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pET28A-blaBIC-1
- 载体抗性:
- Kanamycin
- 载体长度:
- 6196 bp
- 载体类型:
- Cloning vector
- 复制子:
- ori
- 载体来源:
- Hu XM, Yan XH, Yang B, Wang Q
pET28A-blaBIC-1 载体载体图谱
pET28A-blaBIC-1 载体载体序列
LOCUS 62056_9895 6196 bp DNA circular SYN 06-MAY-2019 DEFINITION Cloning vector pET28A-blaBIC-1, complete sequence. ACCESSION MK847898 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6196) AUTHORS Hu XM, Yan XH, Yang B, Wang Q, Zheng L. TITLE Full-length Real-time PCR for Rapid Detection of Seventeen Carbapenemase Genes in Gram-negative bacteria JOURNAL Unpublished REFERENCE 2 (bases 1 to 6196) AUTHORS Hu XM, Yan XH, Yang B, Wang Q, Zheng L. TITLE Direct Submission JOURNAL Submitted (25-APR-2019) Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, No. 1383, Guangzhou Dadao Bei, Guangzhou, Guangdong 510515, China REFERENCE 3 (bases 1 to 6196) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (25-APR-2019) Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, No. 1383, Guangzhou Dadao Bei, Guangzhou, Guangdong 510515, China" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..6196 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 12..467 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(563..1375) /label=KanR /note="aminoglycoside phosphotransferase" rep_origin 1497..2085 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(2271..2413) /label=bom /note="basis of mobility region from pBR322" CDS complement(2518..2706) /label=rop /note="Rop protein, which maintains plasmids at low copy number" protein_bind complement(3481..3502) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS complement(3518..4597) /label=lacI /note="lac repressor" promoter complement(4598..4675) /label=lacI promoter promoter 4984..5002 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 5003..5027 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 5042..5064 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 5083..5100 /label=6xHis /note="6xHis affinity tag" CDS 5110..5127 /label=thrombin site /note="thrombin recognition and cleavage site" gene 5140..6024 /gene="blaBIC" /allele="blaBIC-1" /label=blaBIC CDS 5140..6024 /codon_start=1 /transl_table=11 /gene="blaBIC" /allele="blaBIC-1" /product="carbapenem-hydrolyzing class A beta-lactamase BIC-1" /label=blaBIC /protein_id="QCH41449.1" /translation="MARPSKLALSFSLLLPFLPFTSFAETWPQGDIARQKIVKLEKDFG GRIGVSAIDTGANRTFDFRADERFPLCSSFKGFLAGAVLSHSQQQEGLLEKRIDYKNRV MEPHSPISAQHSSTGMTVAQLAAAALQYSDNGATNLLLENVLGGPAGMTTFMRTLGDTT FRLDRWELELNSAIPGDDRDTSTPHAIARSLQKIALGEALQTAPRQQLVDWLIGNTTGG ARIRAGVPVEWVVGDKTGTCGVYGTANDYAVIWPKTSAPIVLAIYTAKPNKEDKHSDAV IAEVTRAVLESFE" CDS 6040..6057 /label=6xHis /note="6xHis affinity tag" terminator 6124..6171 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase"
我们不提供该载体,载体信息仅供查询和参考。