pBG51 载体 (V009211#)

载体基本信息
载体名称 pBG51 载体抗性 Kanamycin
载体长度 3959 bp 载体类型 Cloning vector
载体来源 Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM.

pBG51 载体载体图谱

pBG513959 bp60012001800240030003600promoter 14dBCD2 bicistronic linkermsfGFPMCST0 transcriptional terminatorTn7R transposase recognition siteaphAoriT origin of transferR6KTn7L transposase recognition siteT1 transcriptional terminatoraacC1Pc promoter

pBG51 载体载体序列

LOCUS       Exported                3959 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBG51, complete sequence.
ACCESSION   KT192133
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank 
            LM.
  TITLE     A Tn7-based device for calibrated heterologous gene expression in 
            Pseudomonas putida
  JOURNAL   ACS Synth Biol (2015) In press
  PUBMED    26133359
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank 
            LM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2015) System Biology, Centro Nacional de 
            Biotecnologia, C/Darwin 3, Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3959)
  AUTHORS   Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank 
            LM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2015) System Biology, Centro Nacional de 
            Biotecnologia, C/Darwin 3, Madrid 28049, Spain
REFERENCE   4  (bases 1 to 3959)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Nov 13, 2015 this sequence version replaced KT192133.1.
            ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Cloning vector pBG51"
                     /mol_type="other DNA"
                     /note="derived from cloning vector pSEVA211 as backbone to 
                     clone mini-Tn7 fragments bearing Tn7 extremes, 
                     multi-cloning site, and in some cases reporter (gfp) and 
                     synthetic promoters"
                     /db_xref="taxon:1673858"
     regulatory      9..49
                     /regulatory_class="promoter"
                     /note="promoter 14d"
     misc_feature    56..132
                     /label=BCD2 bicistronic linker
                     /note="BCD2 bicistronic linker"
     RBS             124..132
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             139..867
                     /codon_start=1
                     /gene="msfgfp"
                     /product="msfGFP"
                     /label=msfgfp
                     /note="monomeric superfolder green fluorescent protein"
                     /protein_id="AKQ70990.1"
                     /translation="MIMGIHKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGK
                     LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISF
                     KDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKN
                     GIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK"
     gene            139..867
                     /gene="msfgfp"
                     /label=msfgfp
                     /note="monomeric superfolder green fluorescent protein"
     misc_feature    868..924
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      963..1065
                     /regulatory_class="terminator"
                     /note="T0 transcriptional terminator"
     terminator      967..1061
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    1066..1264
                     /label=Tn7R transposase recognition site
                     /note="Tn7R transposase recognition site"
     CDS             1374..2189
                     /codon_start=1
                     /gene="aphA"
                     /product="aminoglycoside 3'-phosphotransferase"
                     /label=aphA
                     /note="phosphotransferase; kanamycin resistance marker"
                     /protein_id="AKQ70991.2"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            1374..2189
                     /gene="aphA"
                     /label=aphA
                     /note="kanamycin resistance marker"
     misc_feature    2210..2455
                     /label=oriT origin of transfer
                     /note="oriT origin of transfer"
     oriT            2345..2453
                     /note="incP origin of transfer"
     rep_origin      2472..2863
                     /label=R6K
                     /note="R6K"
     rep_origin      2472..2860
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     misc_feature    2872..3037
                     /label=Tn7L transposase recognition site
                     /note="Tn7L transposase recognition site"
     mobile_element  2872..3037
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     regulatory      3038..3142
                     /regulatory_class="terminator"
                     /note="T1 transcriptional terminator"
     terminator      3051..3137
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3203..3736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="3'-N-acetyltransferase"
                     /label=aacC1
                     /note="gentamicin resistance marker"
                     /protein_id="AKQ70992.1"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     gene            complement(3203..3736)
                     /gene="aacC1"
                     /label=aacC1
                     /note="gentamicin resistance marker"
     promoter        complement(3925..3953)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"

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