载体基本信息 | |||
载体名称 | pBG51 | 载体抗性 | Kanamycin |
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载体长度 | 3959 bp | 载体类型 | Cloning vector |
载体来源 | Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM. |
pBG51 载体载体图谱
pBG51 载体载体序列
LOCUS Exported 3959 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pBG51, complete sequence. ACCESSION KT192133 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3959) AUTHORS Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM. TITLE A Tn7-based device for calibrated heterologous gene expression in Pseudomonas putida JOURNAL ACS Synth Biol (2015) In press PUBMED 26133359 REFERENCE 2 (bases 1 to 3959) AUTHORS Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM. TITLE Direct Submission JOURNAL Submitted (09-JUN-2015) System Biology, Centro Nacional de Biotecnologia, C/Darwin 3, Madrid 28049, Spain REFERENCE 3 (bases 1 to 3959) AUTHORS Zobel S, Benedetti I, Eisenbach L, de Lorenzo V, Wierckx N, Blank LM. TITLE Direct Submission JOURNAL Submitted (13-NOV-2015) System Biology, Centro Nacional de Biotecnologia, C/Darwin 3, Madrid 28049, Spain REFERENCE 4 (bases 1 to 3959) AUTHORS . TITLE Direct Submission COMMENT On Nov 13, 2015 this sequence version replaced KT192133.1. ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3959 /organism="Cloning vector pBG51" /mol_type="other DNA" /note="derived from cloning vector pSEVA211 as backbone to clone mini-Tn7 fragments bearing Tn7 extremes, multi-cloning site, and in some cases reporter (gfp) and synthetic promoters" /db_xref="taxon:1673858" regulatory 9..49 /regulatory_class="promoter" /note="promoter 14d" misc_feature 56..132 /label=BCD2 bicistronic linker /note="BCD2 bicistronic linker" RBS 124..132 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 139..867 /codon_start=1 /gene="msfgfp" /product="msfGFP" /label=msfgfp /note="monomeric superfolder green fluorescent protein" /protein_id="AKQ70990.1" /translation="MIMGIHKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGK LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISF KDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKN GIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHM VLLEFVTAAGITHGMDELYK" gene 139..867 /gene="msfgfp" /label=msfgfp /note="monomeric superfolder green fluorescent protein" misc_feature 868..924 /label=MCS /note="pUC18/19 multiple cloning site" regulatory 963..1065 /regulatory_class="terminator" /note="T0 transcriptional terminator" terminator 967..1061 /label=lambda t0 terminator /note="transcription terminator from phage lambda" misc_feature 1066..1264 /label=Tn7R transposase recognition site /note="Tn7R transposase recognition site" CDS 1374..2189 /codon_start=1 /gene="aphA" /product="aminoglycoside 3'-phosphotransferase" /label=aphA /note="phosphotransferase; kanamycin resistance marker" /protein_id="AKQ70991.2" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" gene 1374..2189 /gene="aphA" /label=aphA /note="kanamycin resistance marker" misc_feature 2210..2455 /label=oriT origin of transfer /note="oriT origin of transfer" oriT 2345..2453 /note="incP origin of transfer" rep_origin 2472..2863 /label=R6K /note="R6K" rep_origin 2472..2860 /label=R6K gamma ori /note="gamma replication origin from E. coli plasmid R6K; requires the R6K initiator protein pi for replication" misc_feature 2872..3037 /label=Tn7L transposase recognition site /note="Tn7L transposase recognition site" mobile_element 2872..3037 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" regulatory 3038..3142 /regulatory_class="terminator" /note="T1 transcriptional terminator" terminator 3051..3137 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" CDS complement(3203..3736) /codon_start=1 /gene="aacC1" /product="3'-N-acetyltransferase" /label=aacC1 /note="gentamicin resistance marker" /protein_id="AKQ70992.1" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" gene complement(3203..3736) /gene="aacC1" /label=aacC1 /note="gentamicin resistance marker" promoter complement(3925..3953) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter"
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