我们所展示的质粒图谱主要是从文献和开放数据库中收集而来，主要是为了方便研究工作，其中一小部分质粒进行了质量控制，可供科学家使用。
所有的产品都严格仅供科学研究使用，不能应用于临床试验，包括人体摄入、注射或外用的药理学用途。
确保质粒的关键元件正确，但是我们并不能保证实验结果。页面展示的图谱序列为理论序列，可能与测序结果不一致，请自行比对后确定是否满足要求。(如果测过序，本页面一般会提供下载)
开放数据库中的大多数载体序列都没有被完全测序。如果实际序列与参考序列的相似度超过99%，则将其视为正确。
由于科学研究是在探索未知，具有很大的不确定性，在任何情况下，我们都不承担超出质粒本身的额外经济损失或责任。
- 载体名称:
- pRRL-sffv-eGFP-cmv-hsCDNF
- 载体抗性:
- Ampicillin
- 载体长度:
- 8624 bp
- 载体类型:
- Cloning vector
- 复制子:
- ori
- 载体来源:
- Cordero Llana O.
- 启动子:
- SFFV
pRRL-sffv-eGFP-cmv-hsCDNF 载体载体图谱
pRRL-sffv-eGFP-cmv-hsCDNF 载体载体序列
LOCUS V003621 8624 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V003621
VERSION V003621
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 8624)
AUTHORS Cordero Llana O.
TITLE Direct Submission
JOURNAL Submitted (12-APR-2014) Physiology Anatomy and Genetics, University
of Oxford, South Parks Rd., Oxford, England OX1 3QX, United Kingdom
REFERENCE 2 (bases 1 to 8624)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 8624)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(12-APR-2014) Physiology Anatomy and Genetics, University of Oxford,
South Parks Rd., Oxford, England OX1 3QX, United Kingdom"
SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8624
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 52..640
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
misc_feature 710..725
/label="cPPPT5'"
/note="cPPPT5'"
LTR 727..960
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 1032..1166
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 1172..1307
/label="SV40 ori"
/note="SV40 origin of replication"
promoter complement(1340..1358)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1368..1384)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 1529..1984
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2013..2117
/label="AmpR promoter"
CDS 2118..2975
/label="AmpR"
/note="beta-lactamase"
rep_origin 3149..3737
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4025..4046
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4061..4091
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 4099..4115
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4123..4139
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 4160..4178
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 4206..4433
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
misc_feature 4434..4614
/label="HIV-LTR"
/note="HIV-LTR"
LTR 4434..4614
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 4658..4783
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5276..5509
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 5694..5738
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 5887..5928
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 5999..6116
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 6233..6640
/label="SFFV promoter"
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
CDS 6662..7378
/label="EGFP"
/note="enhanced GFP"
enhancer 7458..7761
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 7762..7965
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 8060..8620
/gene="CDNF"
/label="Cerebral dopamine neurotrophic factor"
/note="Cerebral dopamine neurotrophic factor from Homo
sapiens. Accession#: Q49AH0"
我们不提供该载体，载体信息仅供查询和参考。