| 载体基本信息 | |||
| 载体名称 | pSpiro-PAC-IFT46-3xHA-C | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 5134 bp | 载体类型 | Episomal vector |
| 载体来源 | Jerlstrom-Hultqvist J, Einarsson E, Svard SG. | ||
pSpiro-PAC-IFT46-3xHA-C 载体载体图谱
pSpiro-PAC-IFT46-3xHA-C 载体载体序列
LOCUS Exported 5134 bp ds-DNA circular SYN 18-DEC-2018
DEFINITION Episomal vector pSpiro-PAC-IFT46-3xHA-C, complete sequence.
ACCESSION JQ768101
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5134)
AUTHORS Jerlstrom-Hultqvist J, Einarsson E, Svard SG.
TITLE Stable Transfection of the Diplomonad Parasite Spironucleus
salmonicida
JOURNAL Eukaryotic Cell 11 (11), 1353-1361 (2012)
PUBMED 22983987
REFERENCE 2 (bases 1 to 5134)
AUTHORS Jerlstroem-Hultqvist JSE., Einarsson EM, Svaerd SG.
TITLE Direct Submission
JOURNAL Submitted (09-MAR-2012) Department of Cell and Molecular Biology,
Uppsala University, BMC, Box 596, Uppsala 751 24, Sweden
REFERENCE 3 (bases 1 to 5134)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5134
/organism="Episomal vector pSpiro-PAC-IFT46-3xHA-C"
/mol_type="other DNA"
/db_xref="taxon:1233395"
promoter 24..128
/gene="bla"
/label=AmpR promoter
CDS 129..989
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS 327..989
/codon_start=1
/transl_except="(pos: 987 .. 989, aa: TERM)"
/product="beta-lactamase"
/label=beta-lactamase
/protein_id="AFS33193.1"
/translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
rep_origin 1160..1748
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2036..2057
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2072..2102
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2110..2126
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2134..2150
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 2171..2189
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
3'UTR complement(2214..2423)
/note="ornithine carbamoyltransferase from Spironucleus
salmonicida"
CDS complement(2421..3029)
/codon_start=1
/product="puromycin N-acetyltransferase"
/label=puromycin N-acetyltransferase
/protein_id="AFS33194.1"
/translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
SAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGALE"
CDS complement(2430..3029)
/codon_start=1
/gene="pac from Streptomyces"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
SAPRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
5'UTR complement(3030..3191)
/note="ornithine carbamoyltransferase from Spironucleus
salmonicida"
5'UTR 3250..3501
/note="intraflagellar transport 46 homolog from
Spironucleus salmonicida"
CDS 3502..4419
/codon_start=1
/product="intraflagellar transport 46-3xHA"
/label=intraflagellar transport 46-3xHA
/protein_id="AFS33195.1"
/translation="MEMVLILILKWGKSEDILLYITKHKY*IFHFKFSMADNHEDDTLT
DEPSDNFATLTNDRLLELFRTFVPTELMLETLFVPFLPDY*PAIGDVDPFIKVGRPDGK
LEPLGLIVLDEPCNNQSEPTILESMLEAKTKPIARSKDKTTIKSNKKDKKSQDAKIKAI
DYDKEIIHDWVDKVEKIH*T*GSAEYNYSVPVPSATILELWDTEMESIIKTIDLPKPSI
ELSIE*YAKVCCLILDIPVGPNAIESLHMFFTNYRDVLDETEVIK*Q*GY*RPPYPYDV
PDYAYPYDVPDYAYPYDVPDYAGP"
CDS 4330..4410
/codon_start=1
/product="three tandem HA epitope tags"
/label=3xHA
/translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
3'UTR 4417..4476
/note="ribosomal protein S15A from Spironucleus
salmonicida"
promoter complement(4491..4509)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4519..4535)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4677..5132
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
我们不提供该载体，载体信息仅供查询和参考。