- 载体名称:
- pFA-TagRFP-T-CmLEU2
- 载体抗性:
- Ampicillin
- 载体长度:
- 4822 bp
- 载体类型:
- Cloning vector
- 复制子:
- ori
- 载体来源:
- Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M.
pFA-TagRFP-T-CmLEU2 载体载体图谱
pFA-TagRFP-T-CmLEU2 载体载体序列
LOCUS 40924_19141 4822 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pFA-TagRFP-T-CmLEU2, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4822) AUTHORS Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M. TITLE Actin dynamics and endocytosis in the absence of Arp2/3 in Candida albicans JOURNAL Unpublished REFERENCE 2 (bases 1 to 4822) AUTHORS Epp E, Nazarova E, Douglas LM, Konopka JB, Vogel J, Whiteway M. TITLE Direct Submission JOURNAL Submitted (24-MAY-2011) Biology, McGill University, 1205 Docteur Penfield, Montreal, Quebec H3A 1B1, Canada REFERENCE 3 (bases 1 to 4822) TITLE Direct Submission REFERENCE 4 (bases 1 to 4822) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (24-MAY-2011) Biology, McGill University, 1205 Docteur Penfield, Montreal, Quebec H3A 1B1, Canada" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4822 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 58..786 /label=TagRFP-T /note="photostable variant of TagRFP (Shaner et al., 2008)" regulatory 792..1045 /label=Saccharomyces cerevisiae URA3 terminator /note="Saccharomyces cerevisiae URA3 terminator" /regulatory_class="terminator" CDS 1214..2332 /gene="LEU2" /label=LEU2 /note="3-isopropylmalate dehydrogenase from Candida maltosa. Accession#: P07139" promoter complement(2460..2478) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(2736..3324) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3498..4355) /label=AmpR /note="beta-lactamase" promoter complement(4356..4460) /label=AmpR promoter promoter 4806..4822 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase"
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