载体基本信息 | |||
载体名称 | pBCGE7 | 载体抗性 | Kanamycin |
---|---|---|---|
载体长度 | 6984 bp | 载体类型 | Expression vector |
载体来源 | Govan VA, Williamson AL. |
pBCGE7 载体载体图谱
pBCGE7 载体载体序列
LOCUS Exported 6984 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Expression vector pBCGE7, complete sequence. ACCESSION EF175995 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6984) AUTHORS Govan VA, Williamson AL. TITLE Rabbits immunised with recombinant BCG expressing the cottontail rabbit papillomavirus (CRPV) L2E7E2 genes induces regression of established papillomas JOURNAL Virus Res. 127 (1), 43-48 (2007) PUBMED 17451831 REFERENCE 2 (bases 1 to 6984) AUTHORS Govan VA, Williamson A-L. TITLE Direct Submission JOURNAL Submitted (10-DEC-2006) Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Anzio Road, Observatory, Cape Town 7925, South Africa REFERENCE 3 (bases 1 to 6984) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..6984 /organism="Expression vector pBCGE7" /mol_type="other DNA" /label=derived from Cottontail rabbit papillomavirus E7 /note="derived from Cottontail rabbit papillomavirus E7" /db_xref="taxon:419255" CDS 1877..2692 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 3024..3612 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS 6556..6879 /codon_start=1 /gene="E7" /product="E7" /label=E7 /protein_id="ABM68359.1" /translation="MIGRTPKLSELVLGETAEALSLHCDEALENLSDDDEEDHQDRQVF IERPYAVSVPCKRCRQTISFVCVCAPEAIRTLNRLLSASLSLVCPECCNLLLLLLIDVD VVN" gene 6556..6879 /gene="E7" /label=E7 terminator 6902..6948 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene"
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