Recombinant Human RNA Demethylase ALKBH5 (Fragment 74-294) is a high-quality, soluble protein fragment designed for structural and biochemical studies. This fragment, derived from the key catalytic domain of ALKBH5, enables researchers to investigate the enzyme's role in N⁶-methyladenosine (m⁶A) RNA demethylation—a critical post-transcriptional regulatory mechanism in eukaryotes. Ideal for in vitro assays, it supports explorations into mRNA processing, translation, and cellular dynamics such as phase separation.

ALKBH5 is an alpha-ketoglutarate-dependent dioxygenase that specifically demethylates N⁶-methyladenosine (m⁶A) in RNA, the most abundant internal modification in messenger RNA. This enzymatic activity requires molecular oxygen, alpha-ketoglutarate, and iron, and it influences mRNA processing, translation, and nuclear export. ALKBH5 is essential for spermatogenesis by ensuring correct mRNA splicing and 3'-UTR length in male germ cells, and it participates in paraspeckle assembly through liquid