Recombinant Angiomotin-like protein 1 (AMOTL1) fragment (residues 432-777) is a high-quality, soluble protein designed for structural and functional studies. This 346-amino acid fragment, derived from human AMOTL1, represents a stable unit ideal for investigating its role in modulating Wnt/β-catenin signaling pathways.

Angiomotin-like protein 1, encoded by the AMOTL1 gene in Homo sapiens, functions as an inhibitor of the Wnt/β-catenin signaling pathway. It achieves this by recruiting CTNNB1 (β-catenin) to recycling endosomes, thereby preventing its nuclear translocation and subsequent transcriptional activation. This regulatory mechanism underscores its importance in cellular processes such as proliferation and differentiation. The protein is localized to tight junctions at cell junctions, highlighting its role in cell-cell adhesion and signaling coordination. The provided fragment (432-777) was computationally selected to ensure stability and solubility, facilitating biochemical and structural analy