Monomethylauristatin F (MMAF) Monoclonal Antibody
- 抗体类型:单克隆
- 抗体来源:
- 抗体应用:ELISA
- 特异性:Chemical
产品详情
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产品名称
Monomethylauristatin F (MMAF) Monoclonal Antibody
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抗体类型
单克隆
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抗体亚型
Mouse IgG2b, κ
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抗体描述
Product has been tested in direct ELISA.
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抗体应用
ELISA
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应用推荐
ELISA: 1:5,000-1:20,000
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特异性
Chemical
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制备方法
MMAF conjugated with BSA. Protein G affinity purified
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组分
PBS(pH7.4), lyophilized. Reconstitution: Adding sterile water, prepare a stock solution of 2.0 mg/ml.
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储存方法
Store at -20℃. Avoid freeze / thaw cycles.
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背景介绍
Auristatins are synthetic analogs of dolastatin 10 (D10), a highly cytotoxic antineoplastic agent employed for cancer chemotherapy, which is derived from a marine species known as Dolabella auricularia. Monomethyl auristatin E (MMAE) and monomethyl auristatin F (MMAF) are identified as the best analogs of dolastatin 10, which bind to microtubules and prevent cell proliferation by inhibiting mitosis. MMAF and MMAE have their advantages and disadvantages. MMAE is more membrane-permeable and has a lower IC50 than MMAF. However, MMAF is more hydrophilic and has a lower aggregation tendency to show lower systemic toxicity than MMAE.
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参考文献
- Lee JW, Stone RL, Lee SJ, Nam EJ, Roh JW, Nick AM, Han HD, Shahzad MM, Kim HS, Mangala LS, Jennings NB, Mao S, Gooya J, Jackson D, Coleman RL, Sood AK. EphA2 targeted chemotherapy using an antibody drug conjugate in endometrial carcinoma. Clin Cancer Res. 2010 May 1;16(9):2562-70. doi: 10.1158/1078-0432.CCR-10-0017. Epub 2010 Apr 13. PMID: 20388851; PMCID: PMC3955167.
- Lee JJ, Choi HJ, Yun M, Kang Y, Jung JE, Ryu Y, Kim TY, Cha YJ, Cho HS, Min JJ, Chung CW, Kim HS. Enzymatic prenylation and oxime ligation for the synthesis of stable and homogeneous protein-drug conjugates for targeted therapy. Angew Chem Int Ed Engl. 2015 Oct 5;54(41):12020-4. doi: 10.1002/anie.201505964. Epub 2015 Aug 28. PMID: 26315561.
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