BRP/BRINP2-related多肽

H-THRILRRLFNLC-OH

H-Thr-His-Arg-Ile-Leu-Arg-Arg-Leu-Phe-Asn-Leu-Cys-OH

12

95.33

C₆₈H₁₁₆N₂₄O₁₅S

1541.86

固相合成

THRILRRLFNLC is a 12-mer peptide, also known as BRINP2-related peptide (BRP), which was discovered through Peptide Predictor, a computational-functional approach based on prohormone convertase (PCSK) prohormone processing. It is generated by the cleavage of the 78-kDa secreted parent protein BRINP2 by enzymes such as PCSK1. The KK and KR cleavage sites on both sides of it can be recognized by proconvertases, releasing this peptide. Structural prediction indicates that it may exist in an α-helical or linear conformation. BRINP2 is mainly expressed in the brains of mice, humans, and pigs. BRP has been detected in both human cerebrospinal fluid and mouse brains. Its concentration in human cerebrospinal fluid ranges from 700 pM to 3 nM, and it also has a certain concentration in mouse brains. BRP can effectively reduce food intake in mice and pigs and has anti-obesity effects. In mice, it can significantly reduce food intake, and this effect can be observed in both male and female mice, as well as in different obesity states (such as lean mice and diet-induced obese mice). In pigs, it can also reduce food intake. Its mechanism of action is independent of known appetite-regulating pathways such as GLP1R, leptin, and MC4R. Instead, it activates the central CREB-FOS signaling pathway, specifically by activating some regions of the hypothalamus (such as the dorsomedial hypothalamic nucleus, preoptic hypothalamic nucleus, tuberal nucleus, and arcuate nucleus, etc.). BRP does not cause changes in pancreatic activity, that is, it does not affect blood glucose levels and insulin secretion. It also does not induce nausea or aversion, showing certain advantages in improving metabolism.

一般情况下， BRP/BRINP2-related多肽 粉末应该保存在-20甚至-70摄氏度. BRP/BRINP2-related多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Coassolo L, B Danneskiold-Samsøe N, Nguyen Q, Wiggenhorn A, Zhao M, Wang DC, Toomer D, Lone J, Wei Y, Patel A, Liparulo I, Kavi D, Wat LW, Reghupaty SC, Kim JJ, Asemi T, Bielczyk-Maczynska E, Li VL, Moya-Garzon MD, Krentz NAJ, Stahl A, Chou DH, Luo L, Svensson KJ. Prohormone cleavage prediction uncovers a non-incretin anti-obesity peptide. Nature. 2025 Mar 5. doi: 10.1038/s41586-025-08683-y. Epub ahead of print. PMID: 40044869.

三氟乙酸（TFA）是一种强酸，常用于从固相树脂中裂解合成的多肽，也可用于提高多肽纯化步骤中的HPLC性能。默认情况下，定制的多肽是以冻干的TFA盐的形式交付的，其中TFA的含量可能高达10-45%。

定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如，nM浓度的TFA已被证明会干扰细胞实验，在某些情况下会抑制细胞增殖，而在其他情况下会增加细胞活力。

TFA去除服务推荐用于:

• 将用于细胞试验的多肽
• 将被用作原料药或制成品的多肽
• 含有大量碱性残基的亲水多肽

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。