Recombinant Human Cholesterol 24-hydroxylase (CYP46A1) Fragment (aa 24-500) – a highly purified, stable protein fragment ideal for investigating cholesterol metabolism and neuronal homeostasis. This 477-amino acid construct retains key functional regions, enabling robust biochemical and structural studies.

Cholesterol 24-hydroxylase (CYP46A1) is a cytochrome P450 monooxygenase critical for maintaining cholesterol homeostasis in the brain. It primarily catalyzes the 24S-hydroxylation of cholesterol, facilitating its elimination from neurons and activating downstream pathways involved in synaptic function and neurosteroid synthesis. The enzyme localizes to endoplasmic reticulum and microsomal membranes, with presence in synaptic and dendritic regions, underscoring its role in neuronal lipid signaling. This recombinant fragment provides a soluble, functional unit for exploring its catalytic mechanisms and regulatory functions in neurological health and disease.