Recombinant DENN domain-containing protein 2D (DENND2D) fragment (amino acids 52-471) from Homo sapiens, encompassing the complete uDENN, cDENN, and dDENN functional domains. This high-purity fragment serves as an essential tool for investigating the guanine nucleotide exchange factor (GEF) activity and Rab protein regulation mechanisms in cellular processes.

The full-length DENN domain-containing protein 2D, encoded by the DENND2D gene, functions as a guanine nucleotide exchange factor (GEF) that activates RAB9A and RAB9B by promoting GDP-to-GTP exchange, thereby converting inactive GDP-bound Rab proteins into their active GTP-bound forms. This activity is crucial for intracellular trafficking and membrane dynamics. The protein is localized primarily in the cytoplasm, as supported by experimental evidence. The provided fragment (52-471) strategically includes all key functional domains (uDENN, cDENN, dDENN), enabling focused studies on domain-specific roles in Rab protein activation and related cell