Recombinant Human Prolyl Hydroxylase EGLN2 (EC 1.14.11.29) Fragment (aa 169-407) is a high-purity protein fragment encompassing the essential Fe2OG dioxygenase domain. This product is an invaluable tool for investigating oxygen-sensing mechanisms, HIF-alpha regulation, and prolyl hydroxylation in normoxic and hypoxic conditions, offering researchers a focused reagent for domain-specific functional studies.

EGLN2, also known as HIF-prolyl hydroxylase 1 (PHD1), is a crucial cellular oxygen sensor that catalyzes the hydroxylation of proline residues in target proteins under normoxic conditions. It primarily targets hypoxia-inducible factor alpha subunits (HIF1A and HIF2A) via recognition of LXXLAP motifs, promoting their degradation via the von Hippel-Lindau ubiquitination pathway. This regulation is vital for hypoxia tolerance, apoptosis in muscle tissues, and neuronal survival. Beyond HIF signaling, EGLN2 hydroxylates diverse substrates including ATF4, IKBKB, and CEP192, linking oxygen sensing to NF-k