HAVDI 多肽

Fmoc-HAVDI-OH

Fmoc-His-Ala-Val-Asp-Ile-OH

5

95.68

C₃₉H₄₉N₇O₁₀

775.84

固相合成

HAVDI polypeptide, a synthetic peptide engineered to mimic the functional properties of the N - cadherin protein, is designed with a precise sequence to actively interact with cells and modulate their behavior. This peptide exhibits the remarkable ability to self - assemble into nanofibrous structures, thereby creating a three - dimensional scaffold that bears a striking resemblance to the natural extracellular matrix (ECM). This scaffold not only offers essential physical support to cells but also plays a crucial role in guiding their growth, adhesion, and proliferation processes.
The specifically designed N - cadherin mimetic peptides, namely Fmoc - HAVDI and Nap - HAVDI, exemplify this self - assembly property. At physiological pH, they spontaneously form a nanofibrous network, which gives rise to a bioactive peptide hydrogel. The nanofibrous network within these pentapeptide hydrogels closely mimics the topology of the natural ECM, and interestingly, the mechanical strength of the gels precisely matches that of the native ECM of neural cells. This unique combination of properties enables the hydrogels to effectively support cell adhesion and proliferation of both neural and non - neural cell lines, thus highlighting the versatility and adaptability of these peptidic scaffolds.
In the realm of tissue engineering, HAVDI polypeptide has emerged as a highly promising candidate. It actively promotes cell - cell interactions, a fundamental process for tissue regeneration. Its biocompatibility with a diverse range of cell types, including both neural and non - neural cells, is a significant advantage, ensuring minimal cytotoxicity and maximizing its potential for use in vivo. This peptide's distinctive characteristics render it an invaluable tool for in - depth studies of cell behavior and for the development of novel, innovative therapies in the field of regenerative medicine. By leveraging its properties, it becomes possible to engineer functional tissues and organs, opening up new avenues and possibilities for the treatment of a wide spectrum of diseases and injuries. Moreover, the tunable nature of the HAVDI polypeptide allows for customization to meet the specific requirements of different applications, further augmenting its versatility and effectiveness in various biomedical contexts.

一般情况下， HAVDI 多肽 粉末应该保存在-20甚至-70摄氏度. HAVDI 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Kaur H, Roy S. Designing aromatic N-cadherin mimetic short-peptide-based bioactive scaffolds for controlling cellular behaviour. J Mater Chem B. 2021 Aug 7;9(29):5898-5913. doi: 10.1039/d1tb00598g. Epub 2021 Jul 15. PMID: 34263278.

三氟乙酸（TFA）是一种强酸，常用于从固相树脂中裂解合成的多肽，也可用于提高多肽纯化步骤中的HPLC性能。默认情况下，定制的多肽是以冻干的TFA盐的形式交付的，其中TFA的含量可能高达10-45%。

定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如，nM浓度的TFA已被证明会干扰细胞实验，在某些情况下会抑制细胞增殖，而在其他情况下会增加细胞活力。

TFA去除服务推荐用于:

• 将用于细胞试验的多肽
• 将被用作原料药或制成品的多肽
• 含有大量碱性残基的亲水多肽

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。