重组人BTC蛋白

人

人胚胎肾细胞（HEK293）

Met 1 - Tyr 111

A DNA sequence encoding the human BTC (P35070) (Met1-Tyr111) was expressed with six amino acids (LEVLFQ) at the C-terminus.

> 90 % as determined by SDS-PAGE

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.
The ED₅₀ for this effect is typically 0.2-1 ng/mL.

The recombinant human BTC comprises 87 amino acids and has a predicted molecular mass of 9.8 kDa. The apparent molecular mass of the protein is approximately 24 kDa in SDS-PAGE under reducing conditions.

PROBETACELLULIN; BCN; BTC; BETACELLULIN

Probetacellulin

Betacellulin(BTC) is a member of the epidermal growth factor (EGF) family. These soluble proteins are ligands for one or more of the four receptor tyrosine kinases encoded by the ErbB gene family (ErbB-1/epidermal growth factor receptor (EGFR), neu/ErbB-2/HER2, ErbB-3/HER3 and ErbB-4/HER4). Betacellulin is a 32-kilodalton glycoprotein that appears to be processed from a larger transmembrane precursor by proteolytic cleavage. This protein is a ligand for the EGF receptor. BTC is a polymer of about 62-111 amino acid residues. Secondary Structure: 6% helical (1 helices; 3 residues)36% beta sheet (5 strands; 18 residues). BTC was originally identified as a growth-promoting factor in mouse pancreatic β-cell carcinoma cell line and has since been identified in humans. It plays a role in the growth and development of the neonate and/or mammary gland function. Betacellulin is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Lyophilized from sterile PBS, pH 7.4.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.