重组人CD80蛋白
- 蛋白种属:人
- 表达宿主:人胚胎肾细胞(HEK293)
- Unitprot ID:P33681
产品详情
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产品名称
重组人CD80蛋白
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蛋白种属
人
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表达宿主
人胚胎肾细胞(HEK293)
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Unitprot ID
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蛋白序列
Met 1 - Asn 242
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克隆构建
A DNA sequence encoding the extracellular domain (Met 1-Asn 242) of human B7-1 (NP_005182.1) precursor was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.
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蛋白纯度
> 95 % as determined by SDS-PAGE
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蛋白活性
1. Measured by its ability to bind human CD28 in a functional ELISA.
2. Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells.
The ED50 for this effect is 0.2-0.5μg/mL
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分子量
The recombinant human B7-1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 455 amino acids and predicts a molecular mass of 51.7 kDa. By SDS-PAGE, the apparent molecular mass of rh B7-1/Fc is approximately 80-90 kDa due to the glycosylation.
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蛋白别名
B7; TSA1; B71; CD28LG1; CD28LG; B7.1; B7-1; LAB7; LY-53; MIC17; LY53; BB1; CD80; CD28L
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蛋白全名
CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), isoform CRA_a
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蛋白描述
The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
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发货形式
Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.
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内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
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保存条件
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
重组蛋白定制服务:
纽普生物(NovoPro)提供大肠杆菌、哺乳细胞、昆虫细胞和酵母细胞重组蛋白定制服务
声明:本公司出售产品只能用于科研目的,不得用于诊断或者治疗!