重组人DDR1蛋白
- 蛋白种属:人
- 表达宿主:杆状病毒 - 昆虫细胞
- Unitprot ID:Q08345
产品详情
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产品名称
重组人DDR1蛋白
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蛋白种属
人
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表达宿主
杆状病毒 - 昆虫细胞
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Unitprot ID
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蛋白序列
Arg 444 - Val 913
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克隆构建
A DNA sequence encoding the human DDR1 (Q08345-1) (Arg444-Val913) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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蛋白纯度
> 89 % as determined by SDS-PAGE
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蛋白活性
The specific activity was determined to be 2.75 nmol/min/mg using synthetic AXLtide peptide(CKKSRGDYMTMQIG) as substrate.
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分子量
The recombinant human DDR1 /GST chimera consists of 707 amino acids and has a calculated molecular mass of 80 kDa. The recombinant protein migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
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蛋白别名
NEP; CD167A; TRKE; NTRK4; DDR; AI323681; HGK2; DAAP-278B20.1; CAK; EDDR1; PTK3; 6030432F18; DDR1; MCK10; RTK6; CD167; PTK3A
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蛋白全名
Epithelial discoidin domain-containing receptor 1
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蛋白描述
Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.
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发货形式
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.
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内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
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保存条件
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
重组蛋白定制服务:
纽普生物(NovoPro)提供大肠杆菌、哺乳细胞、昆虫细胞和酵母细胞重组蛋白定制服务
声明:本公司出售产品只能用于科研目的,不得用于诊断或者治疗!