Human fibrinopeptide B (hFpB)

Pyr-GVNDNEEGFFSAR-OH

Pyr-Gly-Val-Asn-Asp-Asn-Glu-Glu-Gly-Phe-Phe-Ser-Ala-Arg-OH

14

95.69

C₆₆H₉₃N₁₉O₂₅

1552.55

36204-23-6

16132131

固相合成

Human fibrinopeptide B (hFpB) is a peptide of 14 amino acids that is released from the amino-terminus of the Bβ-chains of fibrinogen by thrombin. It is also contained within the primary plasmin cleavage product of fibrinogen, Bβ1-42. hFpB stimulates the migration of PMN, with a peak effect occurring at 10^-8 M. This chemotactic activity is blocked by preincubating hFpB with antiserum to hFpB. PMN chemotaxis to hFpB does not involve the receptors for C5a, LTB4, or fMLP. hFpB also exerts chemotactic activity for fibroblasts, and its potency is comparable to that observed with PDGF under identical test conditions. hFpB causes an increase in cytoskeletal-associated actin in PMN within 30 s, showing a clear dose response with the maximal effect at 10^-8 M. However, it does not cause PMN aggregation, release of lysosomal enzymes (lysozyme and β-glucuronidase), or the production of superoxide anion. The chemotactic potency of hFpB for PMN is equivalent to that of C5a, LTB4, and fMLP, and its chemotactic activity for PMN occurs in the same concentration range as these other well-defined PMN chemoattractants.

一般情况下， Human fibrinopeptide B (hFpB) 粉末应该保存在-20甚至-70摄氏度. Human fibrinopeptide B (hFpB) 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Senior RM, Skogen WF, Griffin GL, Wilner GD. Effects of fibrinogen derivatives upon the inflammatory response. Studies with human fibrinopeptide B. J Clin Invest. 1986 Mar;77(3):1014-9. doi: 10.1172/JCI112353. PMID: 3005361; PMCID: PMC423507.

三氟乙酸（TFA）是一种强酸，常用于从固相树脂中裂解合成的多肽，也可用于提高多肽纯化步骤中的HPLC性能。默认情况下，定制的多肽是以冻干的TFA盐的形式交付的，其中TFA的含量可能高达10-45%。

定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如，nM浓度的TFA已被证明会干扰细胞实验，在某些情况下会抑制细胞增殖，而在其他情况下会增加细胞活力。

TFA去除服务推荐用于:

• 将用于细胞试验的多肽
• 将被用作原料药或制成品的多肽
• 含有大量碱性残基的亲水多肽

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。