重组人RPS6KB1蛋白

人

杆状病毒 - 昆虫细胞

Met 1 - Leu 525

A DNA sequence encoding the human RPS6KB1 (P23443-Alpha I) (Met1-Leu525) was fused with the GST tag at the N-terminus.

> 90 % as determined by SDS-PAGE

No Kinase Activity

The recombinant human RPS6KB1 consists of 750 amino acids and predicts a molecular mass of 85.4 KDa. The apparent molecular mass of the protein is approximately 96 Kda in SDS-PAGE under reducing conditions due to glycosylation.

STK14A; P70 RIBOSOMAL S6 KINASE ALPHA; P70S6K1; 4732464A07RIK; S6K; ALPHA 1; 70 KDA RIBOSOMAL PROTEIN S6 KINASE 1; 2610318I15RIK; PS6K; AI314060; AI256796; S6K1; POLYPEPTIDE 1; P70/85S6K; P70 S6K-ALPHA; P70 S6 KINASE ALPHA; 70KDA; RIBOSOMAL PROTEIN S6 KINASE BETA-1; RIBOSOMAL PROTEIN S6 KINASE I; P70S6K; ALPHA 2; SERINE/THREONINE-PROTEIN KINASE 14A; SERINE/THREONINE KINASE 14 ALPHA; P70 S6KA; S6K-BETA-1; P70-S6K; RP23-467J12.2; P70(S6K)-ALPHA; 70KD; P70 S6 KINASE; AA959758; RIBOSOMAL PROTEIN S6 KINASE; P70-S6K 1; P70-ALPHA

PS6K, also known as RPS6KB1, is a serine/threonine-protein kinase. It belongs to the RSK (ribosomal s6 kinase) family. Members of this family function in signal transduction. PS6K is an isoform of p70 ribosomal S6 kinase (S6K). S6K can be activated by mitogenic stimuli such as growth factors, insulin and cytokines. It phosphorylates the ribosomal protein S6. PS6K also phosphorylates other proteins such as elF4B, eEF2K and SKAR. It is a crucial effector of mTOR(rapamycin) signaling. PS6K is dissociated from the EIF3 complex and activated upon mitogenic stimulation, phosphorylation by the mammalian target of mTOR complex 1 (mTORC1). Its active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. PS6K also functions in cell proliferation, cell growth and cell cycle progression.

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 1mM GSH
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.