重组人PYM1蛋白

人

大肠杆菌

A DNA sequence encoding the Human PYM1/PYM/WIBG Met1-Leu204 is expressed with a 6His tag at the C-terminus.

>95% as determined by SDS-PAGE.

PYM; WIBG

PYM homolog 1, exon junction complex associated factor(PYM1)

Partner of Y14 and Mago (WIBG) is a key regulator of the Exon Junction Complex (EJC). EJC is a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs, is a positional landmarker for the intron exon structure of genes, and directs post-transcriptional processes in the cytoplasm, for instance mRNA export, nonsense-mediated mRNA decay or translation. WIBG is a cytoplasmic RNA-binding protein, it can be excluded from nucleus by Crm1. WIBG as a cooperateing partner of Mago-14, relates with Mago-14 by its N-terminal domain.

Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 100mM NaCl, 10% Glycerol, pH 8.0.

<1.0 EU per μg protein as determined by the LAL method.

Store at <-20°C, minimize freeze-thaw cycles.