IGF-1bsn 多肽

Nap-FFGGYGSSSRRAPQT

1-Nap-Phe-Phe-Gly-Gly-Tyr-Gly-Ser-Ser-Ser-Arg-Arg-Ala-Pro-Gln-Thr-OH

15

95.45

C₈₃H₁₁₂N₂₂O₂₃

1785.91

固相合成

The peptide IGF-1bsn (Insulin-like growth factor-1 bioactive supramolecular nanofiber) is a novel biomaterial. It is obtained by covalently linking dodecapeptides (GYGSSSRRAPQT), which are a key binding site of IGF-1 receptor (IGF-1R) and play an important role in regulating IGF-1 activity, to an assembly peptide, Nap-FFG. IGF-1bsn has the potential to self-assemble and can form an opaque hydrogel by the heating-cooling process in PBS buffer (pH 7.4) at a concentration of 1 mmol/L.
The microstructure of IGF-1bsn is a three-dimensional network composed of nanofibers with a diameter of approximately 20 nm, and Fourier transform infrared spectroscopy shows the characteristic peaks of an amide I band at 1638 cm⁻¹ and an amide III absorption band at approximately 1232 cm⁻¹, corresponding to hydrogen-bonded β-sheet secondary structures.
IGF-1bsn exhibits good stability under physiological conditions and has weak frequency dependence, with the storage modulus (G′) being approximately an order of magnitude higher than the loss modulus (G″). In vitro degradation shows that IGF-1bsn remains above 26% after 24 h.
In rabbit BMSCs experiments, IGF-1bsn promotes BMSC proliferation in a mild and slow manner, enhances the migration of BMSCs at a concentration of 200 nM in vitro, and inhibits BMSC apoptosis. The optimal range to promote cell proliferation and migration, and to inhibit cell apoptosis is 100-200 nM, and 100 times of this concentration (10 μM) is used in subsequent in vivo experiments.
In summary, IGF-1bsn has good stability, biological activity, and the potential to enhance the activity of BMSCs, playing a role in osteogenic / chondrogenic differentiation of BMSCs to improve osteochondral healing and gap space integration after AOT mosaicplasty.

一般情况下， IGF-1bsn 多肽 粉末应该保存在-20甚至-70摄氏度. IGF-1bsn 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Wu H, Shang Y, Sun W, Ouyang X, Zhou W, Lu J, Yang S, Wei W, Yao X, Wang X, Zhang X, Chen Y, He Q, Yang Z, Ouyang H. Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl (BSN-GelMA) hydrogel. Bioact Mater. 2022 Apr 5;19:88-102. doi: 10.1016/j.bioactmat.2022.03.038. PMID: 35441114; PMCID: PMC9005961.

三氟乙酸（TFA）是一种强酸，常用于从固相树脂中裂解合成的多肽，也可用于提高多肽纯化步骤中的HPLC性能。默认情况下，定制的多肽是以冻干的TFA盐的形式交付的，其中TFA的含量可能高达10-45%。

定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如，nM浓度的TFA已被证明会干扰细胞实验，在某些情况下会抑制细胞增殖，而在其他情况下会增加细胞活力。

TFA去除服务推荐用于:

• 将用于细胞试验的多肽
• 将被用作原料药或制成品的多肽
• 含有大量碱性残基的亲水多肽

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。