重组人IL17F蛋白

人

人胚胎肾细胞（HEK293）

Arg 31 - Gln 163

A DNA sequence encoding the human IL17F (NP_443104.1) (Arg 31-Gln 163) was expressed, with a polyhistidine tag at the N-terminus.

> 80 % as determined by SDS-PAGE

1. Measured by its ability to bind human IL17Ra in a functional ELISA.
2. Measured by its ability to induce IL-6 secretion by HFF human foreskin fibroblast cells. The ED₅₀ for this effect is typically 0.5-3 μg/mL.

The recombinant human IL17F consists of 153 amino acids and has a calculated molecular mass of 17.3 kDa. rhIL17F migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions and a 44 kDa band in non-reduced SDS-PAGE as the homodimer.

IL17F; ML1; C87042; ML-1; CANDF6; CYTOKINE ML-1; IL-24; INTERLEUKIN 17F; INTERLEUKIN-24; MUTANT IL-17F; IL-17F

Interleukin-17F

Interleukin-17F (IL-17F) is a cytokine that shares sequence similarity with IL17. The most notable role of IL-17 is it involvement in inducing and mediating proinflammatory responses. IL-17 is commonly associated with allergic responses. IL-17F is expressed by activated T cells, and was expressed only in activated CD4+ T cells and activated monocytes. IL-17F has been shown to stimulate the production of several other cytokines, including IL6 and IL8. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. Recombinant human IL-17F did not stimulate the proliferation of hematopoietic progenitors or the migration of mature leukocytes. However, it markedly inhibited the angiogenesis of human endothelial cells and induced endothelial cells to produce IL-2, TGF-{beta}, and monocyte chemoattractant protein-1. IL-17F stimulates the production of other cytokines and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL-17F stimulates PBMC and T-cell proliferation. It also function in inhibiting angiogenesis By similarity. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.