Melimine 多肽

产品详情

  • 产品名称
    Melimine 多肽
  • 序列
    H-TLISWIKNKRKQRPRVSRRRRRRGGRRRR-OH
  • 三字母序列
    H-Thr-Leu-Ile-Ser-Trp-Ile-Lys-Asn-Lys-Arg-Lys-Gln-Arg-Pro-Arg-Val-Ser-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Gly-Arg-Arg-Arg-Arg-OH
  • 长度 (aa)
    29
  • 多肽纯度 (HPLC, %)
    97.56
  • 分子式
    C158H290N74O35
  • 分子量(Da)
    3786.45
  • 来源
    固相合成
  • 描述
    Melimine and Mel4 are chimeric cationic peptides with broad-spectrum antimicrobial activity. They have been shown to be highly biocompatible in animal models and human clinical trials. The current study examined the mechanism of action of these two antimicrobial peptides against P. aeruginosa. The efect of the peptides of endotoxin neutralization, and their interactions with cytoplasmic membranes using DiSC(3)-5 and Sytox green, Syto-9 and PI dyes were analysed. Release of ATP and DNA/RNA were determined using ATP luminescence and increase in OD260nm. The bacteriolytic ability of the peptides was determined by measuring decreases in OD620nm. Both the peptides neutralized LPS suggesting their interaction with lipid A. Cytoplasmic membrane was disrupted within 30seconds, which correlated with reductions in cellular viability. At 2minutes melimine or Mel4, released 75% and 36% cellular ATP respectively (P<0.001). Membrane permeabilization started 5minutes with simultaneous release of DNA/RNA. Flow cytometry demonstrated 52% and 18% bacteria were stained with PI after 30minutes. Overall, melimine showed higher capacity for membrane disruption compared to Mel4 (P<0.001). The fndings of this study have been summarized as a timeline of bactericidal activity, suggesting that the peptides permeabilized P. aeruginosa within 5minutes, started lysis within 2hours of exposure.
  • 储存指南
    一般情况下， Melimine 多肽 粉末应该保存在-20甚至-70摄氏度. Melimine 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南
  • 参考文献
    • Yasir M, Dutta D, Willcox MDP. Comparative mode of action of the antimicrobial peptide melimine and its derivative Mel4 against Pseudomonas aeruginosa [published correction appears in Sci Rep. 2019 Sep 10;9(1):13267]. Sci Rep. 2019;9(1):7063. Published 2019 May 8. doi:10.1038/s41598-019-42440-2
  • 关于三氟乙酸盐

    三氟乙酸（TFA）是一种强酸，常用于从固相树脂中裂解合成的多肽，也可用于提高多肽纯化步骤中的HPLC性能。默认情况下，定制的多肽是以冻干的TFA盐的形式交付的，其中TFA的含量可能高达10-45%。

    定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如，nM浓度的TFA已被证明会干扰细胞实验，在某些情况下会抑制细胞增殖，而在其他情况下会增加细胞活力。

    TFA去除服务推荐用于:

    • 将用于细胞试验的多肽
    • 将被用作原料药或制成品的多肽
    • 含有大量碱性残基的亲水多肽

  • 关于多肽净含量

    干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。

  • 摩尔浓度计算器

  • 稀释计算器

  • 百分比浓度计算器

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

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多肽合成服务: 纽普生物（NovoPro）提供常规多肽合成、糖修饰多肽合成、稳定同位素标记的多肽合成、磷酸化多肽合成、荧光标记多肽、多肽的BSA/KLH/OVA等载体蛋白的偶联等服务。

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