MP5 多肽

H-STDSTSAPASNLQPASEYS-OH

H-Ser-Thr-Asp-Ser-Thr-Ser-Ala-Pro-Ala-Ser-Asn-Leu-Gln-Pro-Ala-Ser-Glu-Tyr-Ser-OH

19

95.85

C₇₈H₁₂₁N₂₁O₃₅

1912.9

固相合成

MP5 is a 19 - mer MAREMO peptide (STDSTSAPASNLQPASEYS) that has significant impacts on tumor growth and various cellular mechanisms.
Inhibition of Tumor Growth and Metastasis
In the autologous NT193 murine breast cancer model, peritumorally injected MP5 accumulated in tumors in nude mice. It significantly reduced tumor growth, with 30% of treated tumors completely regressing. It also showed potential in inhibiting tumor cell dissemination as indicated by gene expression analysis of lung tissue.
Effects on Cell Proliferation and Apoptosis
MP5 affected cell cycle checkpoint and G2/M checkpoint gene signatures, downregulated mammary stem cell genes, and reduced the number of Ki67 + cells, indicating an impact on tumor cell proliferation.
It increased staining for the apoptosis marker cleaved caspase 3, enhancing tumor cell apoptosis. In vitro, it inhibited NT193 cell viability in a dose - dependent manner and had different effects on cell viability depending on the cell's epithelial or mesenchymal phenotype.
Impact on EMT and TRAIL Cytotoxicity
MP5 treatment enhanced interferon - gamma (IFNγ) and interferon - alpha (IFNα) response genes, upregulated the expression of the IFNγ response gene Tnfsf10 (encoding TRAIL) and the IFNα response gene Tnfrsf10b (encoding DR5).
It induced a mesenchymal - to - epithelial - transition (MET) - like state, enforced an E phenotype in cultured NT193M cells, and sensitized these cells to TRAIL cytotoxicity in a TNC - dependent manner.
Effects on Fibroblasts and Matrix Organization
MP5 caused a profound downregulation of several ECM genes and fibroblast markers, indicating fibroblast depletion and a reduction in matrix organization.
It also downregulated the hallmark angiogenesis gene set, reduced tumor blood vessels, and improved vessel coverage and reduced leakage, suggesting it normalizes the tumor vasculature.
Activation of Immunity
MP5 treatment enriched the antigen processing and cross - presentation gene signature, upregulated MHC class I and II variants and immunoproteasome complex subunits, increased macrophage infiltration and dendritic cell abundance, and enhanced the activity of CD8 + cytotoxic T lymphocytes. It also reduced the expression of M2 - type macrophage marker Mrc1, promoting antitumorigenic macrophage functionality.

一般情况下， MP5 多肽 粉末应该保存在-20甚至-70摄氏度. MP5 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Li C, Kaur A, Pavlidaki A, Spenlé C, Rajnpreht I, Donnadieu E, Salomé N, Molitor A, Carapito R, Wack F, Erne W, Lefebvre O, Averous G, Mitrentsi I, Loustau T, Orend G. Targeting the MAtrix REgulating MOtif abolishes several hallmarks of cancer, triggering antitumor immunity. Proc Natl Acad Sci U S A. 2024 Oct 15;121(42):e2404485121. doi: 10.1073/pnas.2404485121. Epub 2024 Oct 9. PMID: 39382998.

三氟乙酸（TFA）是一种强酸，常用于从固相树脂中裂解合成的多肽，也可用于提高多肽纯化步骤中的HPLC性能。默认情况下，定制的多肽是以冻干的TFA盐的形式交付的，其中TFA的含量可能高达10-45%。

定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如，nM浓度的TFA已被证明会干扰细胞实验，在某些情况下会抑制细胞增殖，而在其他情况下会增加细胞活力。

TFA去除服务推荐用于:

• 将用于细胞试验的多肽
• 将被用作原料药或制成品的多肽
• 含有大量碱性残基的亲水多肽

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。