MP5 多肽

H-STDSTSAPASNLQPASEYS-OH

H-Ser-Thr-Asp-Ser-Thr-Ser-Ala-Pro-Ala-Ser-Asn-Leu-Gln-Pro-Ala-Ser-Glu-Tyr-Ser-OH

19

95.85

C₇₈H₁₂₁N₂₁O₃₅

1912.9

固相合成

MP5 is a 19 - mer MAREMO peptide (STDSTSAPASNLQPASEYS) that has significant impacts on tumor growth and various cellular mechanisms.
Inhibition of Tumor Growth and Metastasis
In the autologous NT193 murine breast cancer model, peritumorally injected MP5 accumulated in tumors in nude mice. It significantly reduced tumor growth, with 30% of treated tumors completely regressing. It also showed potential in inhibiting tumor cell dissemination as indicated by gene expression analysis of lung tissue.
Effects on Cell Proliferation and Apoptosis
MP5 affected cell cycle checkpoint and G2/M checkpoint gene signatures, downregulated mammary stem cell genes, and reduced the number of Ki67 + cells, indicating an impact on tumor cell proliferation.
It increased staining for the apoptosis marker cleaved caspase 3, enhancing tumor cell apoptosis. In vitro, it inhibited NT193 cell viability in a dose - dependent manner and had different effects on cell viability depending on the cell's epithelial or mesenchymal phenotype.
Impact on EMT and TRAIL Cytotoxicity
MP5 treatment enhanced interferon - gamma (IFNγ) and interferon - alpha (IFNα) response genes, upregulated the expression of the IFNγ response gene Tnfsf10 (encoding TRAIL) and the IFNα response gene Tnfrsf10b (encoding DR5).
It induced a mesenchymal - to - epithelial - transition (MET) - like state, enforced an E phenotype in cultured NT193M cells, and sensitized these cells to TRAIL cytotoxicity in a TNC - dependent manner.
Effects on Fibroblasts and Matrix Organization
MP5 caused a profound downregulation of several ECM genes and fibroblast markers, indicating fibroblast depletion and a reduction in matrix organization.
It also downregulated the hallmark angiogenesis gene set, reduced tumor blood vessels, and improved vessel coverage and reduced leakage, suggesting it normalizes the tumor vasculature.
Activation of Immunity
MP5 treatment enriched the antigen processing and cross - presentation gene signature, upregulated MHC class I and II variants and immunoproteasome complex subunits, increased macrophage infiltration and dendritic cell abundance, and enhanced the activity of CD8 + cytotoxic T lymphocytes. It also reduced the expression of M2 - type macrophage marker Mrc1, promoting antitumorigenic macrophage functionality.

一般情况下， MP5 多肽 粉末应该保存在-20甚至-70摄氏度. MP5 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Li C, Kaur A, Pavlidaki A, Spenlé C, Rajnpreht I, Donnadieu E, Salomé N, Molitor A, Carapito R, Wack F, Erne W, Lefebvre O, Averous G, Mitrentsi I, Loustau T, Orend G. Targeting the MAtrix REgulating MOtif abolishes several hallmarks of cancer, triggering antitumor immunity. Proc Natl Acad Sci U S A. 2024 Oct 15;121(42):e2404485121. doi: 10.1073/pnas.2404485121. Epub 2024 Oct 9. PMID: 39382998.

三氟乙酸（Trifluoroacetic acid，简称 TFA）是高效液相色谱（简称 HPLC）纯化过程中常用的反离子。TFA 的存在可能会对肽的净重量、外观及溶解度产生影响，具体如下：

对净重量的影响：TFA 盐会增加产物的总质量。在大多数情况下，肽的含量占总重量的 80% 以上，剩余部分则为 TFA。

对溶解度的影响：TFA 盐通常能提高肽在水溶液中的溶解度。

在生物检测中的影响：对于大多数标准的体外检测，残留的 TFA 水平一般不会造成干扰。但需注意，在高灵敏度的细胞研究或生化研究中，需关注其存在可能带来的影响。

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。