- 序列:KRFKKFFKKLKNSVKKRAKKFFKKPRVIGVSIPF
- 分子式:C202H334N56O38
相关文档
批次之间数据可能略有差异
产品详情
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产品名称
OH-CATH 多肽
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序列
KRFKKFFKKLKNSVKKRAKKFFKKPRVIGVSIPF
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三字母序列
Lys-Arg-Phe-Lys-Lys-Phe-Phe-Lys-Lys-Leu-Lys-Asn-Ser-Val-Lys-Lys-Arg-Ala-Lys-Lys-Phe-Phe-Lys-Lys-Pro-Arg-Val-Ile-Gly-Val-Ser-Ile-Pro-Phe
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长度 (aa)
34
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多肽纯度 (HPLC, %)
95.3
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分子式
C202H334N56O38
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分子量(Da)
4155.19
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来源
固相合成
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描述
Rich in lysines (35%). Active against E. cloacae (MIC 1 ug/ml), E. aerogenes (MIC 4 ug/ml), E. coli (MIC 6-20 ug/ml), S. aureus (MIC 4 ug/ml), and P. aeruginosa PAO1 (MIC 2 ug/ml). Predicted to be helical based on the known structure of human LL-37. OH-CATH30 is obtained by removing the N-terminal 4 residues. OH-CM6 is obtained by further shortening OH-CATH30 by deleting the C-terminal 10 residues followed by mutations. These derivatives are active against indicator and resistant bacterial strains (MIC in the range of 1.5-25 ug/ml). Activity is reduced in the presence of serum. However, the D-form is stable for at least 12 h. These peptides appear to work on membranes as indicated by rapid killing in 60 min (2XMIC) and dye leakage. Both OH-CATH30 and OH-CM6 displayed in vivo efficacy in reducing drug-resistant E. coli burdens in the neutropenic thigh infection animal model:mouse or murine bacteremia model. It also showed protective effects on E. coli induced rabbit urinary tract infection models (animal model:rabbit)( Zhang et al., 2013). The D-form of these peptides is also active in vivo although with higher cytotoxicity ( Li SA et al. 2010). In addition, topical application of OH-CATH30 is efficacious against drug-resistant P. aeruginosa keratitis ( Li SA et al., 2014). Updated 13/4/2012; Feb2013; 3/2014 GW. OH-CATH has Antibacterial activity. OH-CATH was found in king cobra, Ophiophagus hannah.
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储存指南
一般情况下, OH-CATH 多肽 粉末应该保存在-20甚至-70摄氏度. OH-CATH 多肽 溶解后应该-70摄氏度分装保存,避免反复冻融. 了解更多细节,请查阅手册:多肽溶解及保存指南
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参考文献
- Zhao H, Gan TX, Liu XD, et al. Identification and characterization of novel reptile cathelicidins from elapid snakes. Peptides. 2008;29(10):1685-91.
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关于三氟乙酸盐
三氟乙酸(TFA)是一种强酸,常用于从固相树脂中裂解合成的多肽,也可用于提高多肽纯化步骤中的HPLC性能。默认情况下,定制的多肽是以冻干的TFA盐的形式交付的,其中TFA的含量可能高达10-45%。
定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如,nM浓度的TFA已被证明会干扰细胞实验,在某些情况下会抑制细胞增殖,而在其他情况下会增加细胞活力。
TFA去除服务推荐用于:
- 将用于细胞试验的多肽
- 将被用作原料药或制成品的多肽
- 含有大量碱性残基的亲水多肽
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关于多肽净含量
干品多肽的重量中不仅仅包含多肽,还包含有一些非肽的组份,如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比,这个百分比的数值范围很大,可能从50%到90%,取决于纯度、序列以及合成和纯化的方法,不要将肽的净含量和肽的纯度混为一谈,他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比,而肽的净含量是指样品中肽类物质相对于总物质所占的百分比,肽的净含量通常是氨基酸组分分析或紫外分光法测定的,这个信息主要是在一些对肽的浓度很敏感的实验中,对计算肽的浓度是很重要的。
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摩尔浓度计算器
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稀释计算器
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百分比浓度计算器
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
相关服务
多肽合成服务: 纽普生物(NovoPro)提供常规多肽合成、糖修饰多肽合成、稳定同位素标记的多肽合成、磷酸化多肽合成、荧光标记多肽、多肽的BSA/KLH/OVA等载体蛋白的偶联等服务。