CIGB-552 多肽

Ac-HARIKpTFRRlKWKYKGKFW (p=D-Pro, l=D-Leu)

Ac-His-Ala-Arg-Ile-Lys-D-Pro-Thr-Phe-Arg-Arg-D-Leu-Lys-Trp-Lys-Tyr-Lys-Gly-Lys-Phe-Trp

20

94.26

C₁₃₁H₁₉₉N₃₉O₂₃

2689.2

1630763-23-3

固相合成

It has been demonstrated that the peptide L-2—designed via alanine scanning of the Limulus-derived LALF32-51 region—represents a promising candidate for cellular-level intervention, with its cell-penetrating capacity serving as a functionally advantageous property. Through targeted modification of the primary structure of L-2, a second-generation peptide, CIGB-552, was developed. While its cytotoxic activity was evident, the underlying molecular mechanisms remained incompletely characterized. In this investigation, we observed that CIGB-552 elevates the expression of COMMD1, a protein implicated in copper homeostasis, sodium transport, and the NF-κB signaling cascade. Specifically, CIGB-552 promoted ubiquitination of RelA and suppressed NF-κB–mediated antiapoptotic activity, an effect that was abolished upon COMMD1 knockdown. Additionally, CIGB-552 reduced intrinsic antioxidant capacity and triggered peroxidation of proteins and lipids in susceptible cells. Collectively, these findings elucidate key aspects of the mode of action of CIGB-552, offering valuable insights for the development of future bio-active agents aimed at modulating cellular pathways.

一般情况下， CIGB-552 多肽 粉末应该保存在-20甚至-70摄氏度. CIGB-552 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Lugo JM, Tafalla C, Oliva A, et al. Evidence for antimicrobial and anticancer activity of pituitary adenylate cyclase-activating polypeptide (PACAP) from North African catfish (Clarias gariepinus): Its potential use as novel therapeutic agent in fish and humans. Fish Shellfish Immunol. 2019;86:559-570. doi:10.1016/j.fsi.2018.11.056
• Fernu00e1ndez Massu00f3 JR, Oliva Argu00fcelles B, Tejeda Y, et al. The Antitumor Peptide CIGB-552 Increases COMMD1 and Inhibits Growth of Human Lung Cancer Cells. J Amino Acids. 2013;2013:251398. doi:10.1155/2013/251398

三氟乙酸（Trifluoroacetic acid，简称 TFA）是高效液相色谱（简称 HPLC）纯化过程中常用的反离子。TFA 的存在可能会对肽的净重量、外观及溶解度产生影响，具体如下：

对净重量的影响：TFA 盐会增加产物的总质量。在大多数情况下，肽的含量占总重量的 80% 以上，剩余部分则为 TFA。

对溶解度的影响：TFA 盐通常能提高肽在水溶液中的溶解度。

在生物检测中的影响：对于大多数标准的体外检测，残留的 TFA 水平一般不会造成干扰。但需注意，在高灵敏度的细胞研究或生化研究中，需关注其存在可能带来的影响。

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。