PjCLE1 多肽

H-RLAOEGONPEHH-OH

H-Arg-Leu-Ala-Hyp-Glu-Gly-Hyp-Asn-Pro-Glu-His-His-OH

12

95.19

C₅₈H₈₈N₂₀O₂₀

1385.44

固相合成

PjCLE1 is an endogenous peptide hormone in the parasitic plant Phtheirospermum japonicum. It was identified through genomic analysis, with 14 PjCLE genes found in the genome, and PjCLE1/Pjv1-77 showing significant expression differences in haustoria compared to non-infecting roots and high expression in laser-dissected mature haustoria. It is processed and activated by the subtilisin-type protease PjSBT1.2.3. When incubated with PjSBT1.2.3-His, the cleavage site within the variable domain of the prodomain was determined, and the fully processed PjCLE1 peptide was generated.
Functionally, PjCLE1 is sufficient to induce protohaustorium formation even in the absence of a host, similar to the haustorium-inducing factor DMBQ. Co-treatment with PjCLE1 and DMBQ leads to more prolific protohaustoria formation. Although shoots are not strictly required for PjCLE1-induced protohaustoria formation, their presence affects the process. Sucrose availability can influence protohaustorium formation under certain conditions, and mineral nutrients generally reduce haustorium formation. PjCLE1 also affects signal transduction, enhancing DMBQ-induced MAPK phosphorylation and inducing the expression of genes related to haustoriogenesis, such as PjYUC3. RNA-Seq analysis indicates a similar transcriptional response to DMBQ treatment, with co-treatment potentiating the effect. Overall, PjCLE1 appears to play a crucial role in regulating haustorium formation in Phtheirospermum japonicum, potentially acting as an internal signal to stimulate additional protohaustorium formation for efficient resource extraction from hosts.

一般情况下， PjCLE1 多肽 粉末应该保存在-20甚至-70摄氏度. PjCLE1 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Greifenhagen A, Ruwe H, Zimmer V, Messerschmidt J, Bhukya DPN, Kenea HD, Schaller A, Spallek T. The peptide hormone PjCLE1 stimulates haustorium formation in the parasitic plant Phtheirospermum japonicum. Proc Natl Acad Sci U S A. 2024 Oct 15;121(42):e2414582121. doi: 10.1073/pnas.2414582121. Epub 2024 Oct 9. PMID: 39383005; PMCID: PMC11494319.

三氟乙酸（Trifluoroacetic acid，简称 TFA）是高效液相色谱（简称 HPLC）纯化过程中常用的反离子。TFA 的存在可能会对肽的净重量、外观及溶解度产生影响，具体如下：

对净重量的影响：TFA 盐会增加产物的总质量。在大多数情况下，肽的含量占总重量的 80% 以上，剩余部分则为 TFA。

对溶解度的影响：TFA 盐通常能提高肽在水溶液中的溶解度。

在生物检测中的影响：对于大多数标准的体外检测，残留的 TFA 水平一般不会造成干扰。但需注意，在高灵敏度的细胞研究或生化研究中，需关注其存在可能带来的影响。

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。