PjCLE1 多肽

H-RLAOEGONPEHH-OH

H-Arg-Leu-Ala-Hyp-Glu-Gly-Hyp-Asn-Pro-Glu-His-His-OH

12

95.19

C₅₈H₈₈N₂₀O₂₀

1385.44

固相合成

PjCLE1 is an endogenous peptide hormone in the parasitic plant Phtheirospermum japonicum. It was identified through genomic analysis, with 14 PjCLE genes found in the genome, and PjCLE1/Pjv1-77 showing significant expression differences in haustoria compared to non-infecting roots and high expression in laser-dissected mature haustoria. It is processed and activated by the subtilisin-type protease PjSBT1.2.3. When incubated with PjSBT1.2.3-His, the cleavage site within the variable domain of the prodomain was determined, and the fully processed PjCLE1 peptide was generated.
Functionally, PjCLE1 is sufficient to induce protohaustorium formation even in the absence of a host, similar to the haustorium-inducing factor DMBQ. Co-treatment with PjCLE1 and DMBQ leads to more prolific protohaustoria formation. Although shoots are not strictly required for PjCLE1-induced protohaustoria formation, their presence affects the process. Sucrose availability can influence protohaustorium formation under certain conditions, and mineral nutrients generally reduce haustorium formation. PjCLE1 also affects signal transduction, enhancing DMBQ-induced MAPK phosphorylation and inducing the expression of genes related to haustoriogenesis, such as PjYUC3. RNA-Seq analysis indicates a similar transcriptional response to DMBQ treatment, with co-treatment potentiating the effect. Overall, PjCLE1 appears to play a crucial role in regulating haustorium formation in Phtheirospermum japonicum, potentially acting as an internal signal to stimulate additional protohaustorium formation for efficient resource extraction from hosts.

一般情况下， PjCLE1 多肽 粉末应该保存在-20甚至-70摄氏度. PjCLE1 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Greifenhagen A, Ruwe H, Zimmer V, Messerschmidt J, Bhukya DPN, Kenea HD, Schaller A, Spallek T. The peptide hormone PjCLE1 stimulates haustorium formation in the parasitic plant Phtheirospermum japonicum. Proc Natl Acad Sci U S A. 2024 Oct 15;121(42):e2414582121. doi: 10.1073/pnas.2414582121. Epub 2024 Oct 9. PMID: 39383005; PMCID: PMC11494319.

三氟乙酸（TFA）是一种强酸，常用于从固相树脂中裂解合成的多肽，也可用于提高多肽纯化步骤中的HPLC性能。默认情况下，定制的多肽是以冻干的TFA盐的形式交付的，其中TFA的含量可能高达10-45%。

定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如，nM浓度的TFA已被证明会干扰细胞实验，在某些情况下会抑制细胞增殖，而在其他情况下会增加细胞活力。

TFA去除服务推荐用于:

• 将用于细胞试验的多肽
• 将被用作原料药或制成品的多肽
• 含有大量碱性残基的亲水多肽

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。