PTD-DBM 多肽

产品详情

  • 产品名称
    PTD-DBM 多肽
  • 序列
    H-RRRRRRRRGGGGRKTGHQICKFRKC-OH
  • 三字母序列
    H-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Gly-Gly-Gly-Arg-Lys-Thr-Gly-His-Gln-Ile-Cys-Lys-Phe-Arg-Lys-Cys-OH
  • 长度 (aa)
    25
  • 多肽纯度 (HPLC)
    95.2
  • 分子式
    C124H225N61O28S2
  • 分子量
    3082.62
  • 来源
    固相合成
  • 其他信息
    Wnt/β-catenin signaling plays important roles in cutaneous wound healing and dermal fibrosis. However, its regulatory mechanism has not been fully elucidated, and a commercially available wound-healing agent targeting this pathway is desirable but currently unavailable. We found that CXXC-type zinc finger protein 5 (CXXC5) serves as a negative feedback regulator of the Wnt/β-catenin pathway by interacting with the Dishevelled (Dvl) protein. In humans, CXXC5 protein levels were reduced in epidermal keratinocytes and dermal fibroblasts of acute wounds. A differential regulation of β-catenin, α-smooth muscle actin (α-SMA), and collagen I by overexpression and silencing of CXXC5 in vitro indicated a critical role for this factor in myofibroblast differentiation and collagen production. In addition, CXXC5−/− mice exhibited accelerated cutaneous wound healing, as well as enhanced keratin 14 and collagen synthesis. Protein transduction domain (PTD)–Dvl-binding motif (DBM), a competitor peptide blocking CXXC5-Dvl interactions, disrupted this negative feedback loop and activated β-catenin and collagen production in vitro. Co-treatment of skin wounds with PTD-DBM and valproic acid (VPA), a glycogen synthase kinase 3β (GSK3β) inhibitor which activates the Wnt/β-catenin pathway, synergistically accelerated cutaneous wound healing in mice. Together, these data suggest that CXXC5 would represent a potential target for future therapies aimed at improving wound healing.
  • 储存指南
    一般情况下, PTD-DBM 多肽 粉末应该保存在-20甚至-70摄氏度. PTD-DBM 多肽 溶解后应该-70摄氏度分装保存,避免反复冻融. 了解更多细节,请查阅手册:多肽溶解及保存指南
  • 参考文献
    • Lee SH, Kim MY, Kim HY, et al. The Dishevelled-binding protein CXXC5 negatively regulates cutaneous wound healing. J Exp Med. 2015;212(7):1061-1080. doi:10.1084/jem.20141601
  • 关于三氟乙酸盐

    三氟乙酸(TFA)是一种强酸,常用于从固相树脂中裂解合成的多肽,也可用于提高多肽纯化步骤中的HPLC性能。默认情况下,定制的多肽是以冻干的TFA盐的形式交付的,其中TFA的含量可能高达10-45%。

    定制多肽中的TFA可能会导致后续检测数据出现无法解释的差异。例如,nM浓度的TFA已被证明会干扰细胞实验,在某些情况下会抑制细胞增殖,而在其他情况下会增加细胞活力。

    TFA去除服务推荐用于:

    • 将用于细胞试验的多肽
    • 将被用作原料药或制成品的多肽
    • 含有大量碱性残基的亲水多肽

  • 关于多肽净含量

    干品多肽的重量中不仅仅包含多肽,还包含有一些非肽的组份,如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比,这个百分比的数值范围很大,可能从50%到90%,取决于纯度、序列以及合成和纯化的方法,不要将肽的净含量和肽的纯度混为一谈,他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比,而肽的净含量是指样品中肽类物质相对于总物质所占的百分比,肽的净含量通常是氨基酸组分分析或紫外分光法测定的,这个信息主要是在一些对肽的浓度很敏感的实验中,对计算肽的浓度是很重要的。

多肽性质

  • 序列:H-RRRRRRRRGGGGRKTGHQICKFRKC-OH
  • 分子式:C124H225N61O28S2
  • 长度:25
  • 消光系数:0 M-1cm-1
  • GRAVY:-2.15
  • 分子量:3082.62
  • 理论pI:12.77
  • 数据来源:多肽性质计算器

GRAVY = 平均疏水性(grand average of hydropathy)

X: 疏水不带电残基,如 F I L M V W A 和 P

X: 碱性残基,如 R K H

X: 酸性残基,如 D E

X: 极性不带电残基,如 G S T C N Q 和 Y

多肽合成服务: 纽普生物(NovoPro)提供常规多肽合成、糖修饰多肽合成、稳定同位素标记的多肽合成、磷酸化多肽合成、荧光标记多肽、多肽的BSA/KLH/OVA等载体蛋白的偶联等服务

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